Morphology of the enamel organ in the miniature swine.
Morphology of the enamel organ in the miniature swine.
复制标题
小型猪釉质器官的形态学。
DOI：
10.1002/ar.1092300110
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Moreno,EC
中科院分区：
文献类型：
--
作者：
McKee,MD;Aoba,T;Moreno,EC
近年来，猪的牙齿被越来越多地用作研究成体发育的模型。事实上，我们目前对牙釉质形成的大部分知识都来自于对猪牙釉质有机成分和无机成分的生化和物理化学分析。作为之前工作的延伸，作为我们尝试将已知的牙釉质基质和矿物变化与邻近的牙釉质器官形态联系起来的第一步，本研究进行了对猪无变性过程中牙釉质器官发生的形态学事件的描述。采用戊二醛血管灌注固定2周龄的小型猪（minipigs），用Epon树脂包埋乳牙并切片，用光镜和透射电镜观察乳牙釉质发生各发育阶段的细胞。在许多方面，猪的牙釉质器官的形态与以前在其他哺乳动物物种中描述的相似。另一方面，注意到一些特殊性，并在现有数据的背景下讨论了细胞超微结构与牙釉质形成过程中假定的功能。
In recent years, the dentition of the pig has been increasingly used as a model for the study of amelogenesis. Indeed, much of our current knowledge on enamel formation derives from biochemical and physicochemical analyses of the organic and inorganic components, respectively, of porcine enamel. As an extension of this previous work, and as the first step in our attempt to correlate known enamel matrix and mineral changes with adjacent enamel organ morphology, the present study was undertaken to provide a description of the morphological events occurring in the enamel organ during porcine amelogenesis. Two‐week‐old miniature swine (minipigs) were fixed by vascular perfusion with glutaraldehyde, the deciduous teeth present at this age were embedded in Epon resin and sectioned, and the cells of the enamel organ at each of the various developmental stages of amelogenesis were examined by light and transmission electron microscopy. In many respects, the morphology of the porcine enamel organ was similar to that previously described in other mammalian species. On the other hand, several particularities were noted and these are discussed in the context of available data correlating cell ultrastructure with putative function during enamel formation.