40-MHz echocardiography scanner for cardiovascular assessment of mouse embryos
40-MHz echocardiography scanner for cardiovascular assessment of mouse embryos
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DOI：
10.1016/s0301-5629(98)00132-x
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发表时间：
1998-11-01
影响因子：
2.9
通讯作者：
Turnbull, DH
中科院分区：
文献类型：
--
作者：
Aristizábal, O;Christopher, DA;Turnbull, DH
先天性心脏病是由基因缺陷引起的，这种缺陷在胚胎发生的早期阶段就表现出来了。小鼠是哺乳动物胚胎发育研究和越来越多的人类疾病模型的首选动物模型。在小鼠中发现的许多基因对正常的心血管发育至关重要，但对调节心脏发育的潜在机制的理解仍然不完整，部分原因是缺乏测量活体小鼠胚胎血液动力学的方法。我们描述了一种40兆赫的超声扫描仪的发展，它允许图像引导连续波和脉冲多普勒血流测量小鼠胚胎，在子宫内，在关键的早期发育阶段。从小鼠胚胎脐血管、降主动脉和心室获得的多普勒波形展示了该方法的实用性。通过将图像引导超声多普勒与许多可用的小鼠突变体相结合，这种方法应该会对胚胎心血管结构-功能关系产生新的见解。(C) 1998年世界超声医学和生物学联合会。
Congenital heart disease results from genetic defects that are manifested at early stages of embryogenesis. The mouse is the preferred animal model for studies of mammalian embryonic development and for an increasing number of human disease models. A number of genes identified in the mouse are critical for normal cardiovascular development, but an understanding of the underlying mechanisms regulating heart development is still incomplete, in part because of the lack of methods to measure hemodynamics in live mouse embryos. We describe the development of a 40-MHz ultrasound scanner, which allows image-guided continuous-wave and pulsed Doppler blood flow measurements in mouse embryos, in utero, at the critical early developmental stages. Doppler waveforms acquired from mouse embryonic umbilical vessels, descending aorta, and cardiac ventricles are presented to demonstrate the utility of the method. By combining image-guided ultrasound Doppler with the many available mouse mutants, this approach should lead to new insights into embryonic cardiovascular structure-function relationships. (C) 1998 World Federation for Ultrasound in Medicine & Biology.