Monitoring the Switch from Housekeeping to Pathogen Defense Metabolism in Arabidopsis thaliana Using cDNA Arrays*
Monitoring the Switch from Housekeeping to Pathogen Defense Metabolism in Arabidopsis thaliana Using cDNA Arrays*
复制标题
DOI：
10.1074/jbc.m104863200
复制
发表时间：
2002-03
期刊：
影响因子：
--
通讯作者：
M. Scheideler;N. Schlaich;K. Fellenberg;T. Beissbarth;N. Hauser;M. Vingron;A. Slusarenko;J. Hoheisel-J.-Hoheis
中科院分区：
文献类型：
--
作者：
M. Scheideler;N. Schlaich;K. Fellenberg;T. Beissbarth;N. Hauser;M. Vingron;A. Slusarenko;J. Hoheisel-J.-Hoheis
植物通过部署几种防御反应来应对病原体的攻击。一些依赖于预先形成的成分的激活，而另一些依赖于转录活性的变化。使用包含13，000个独特表达序列标签的cDNA阵列，在尝试感染细菌性植物病原体假单胞菌pv.携带无毒基因avrRpt 2的番茄。在浸润后的前24小时内的4个时间点取样，发现10分钟内650个基因的稳态转录水平发生显著变化，7小时内基因表达模式发生巨大变化，涉及代表许多细胞过程的2,000个基因。这种从持家代谢到防御代谢的转变是由于调节和信号传导电路的变化以及植物抵抗病原体攻击对能量和生物合成能力的需求增加。集中我们的详细分析的基因编码酶的糖酵解，克雷布斯循环，戊糖磷酸途径，芳香族氨基酸，苯丙素类化合物的生物合成，乙烯，我们观察到有趣的差异调节模式。此外，我们的数据表明，在代谢领域，如乙醛酸代谢，迄今尚未怀疑是植物防御的组成部分潜在的重要变化。
Plants respond to pathogen attack by deploying several defense reactions. Some rely on the activation of preformed components, whereas others depend on changes in transcriptional activity. Using cDNA arrays comprising 13,000 unique expressed sequence tags, changes in the transcriptome ofArabidopsis thaliana were monitored after attempted infection with the bacterial plant pathogen Pseudomonas syringae pv. tomato carrying the avirulence geneavrRpt2. Sampling at four time points during the first 24 h after infiltration revealed significant changes in the steady state transcript levels of ∼650 genes within 10 min and a massive shift in gene expression patterns by 7 h involving ∼2,000 genes representing many cellular processes. This shift from housekeeping to defense metabolism results from changes in regulatory and signaling circuits and from an increased demand for energy and biosynthetic capacity in plants fighting off a pathogenic attack. Concentrating our detailed analysis on the genes encoding enzymes in glycolysis, the Krebs cycle, the pentose phosphate pathway, the biosynthesis of aromatic amino acids, phenylpropanoids, and ethylene, we observed interesting differential regulation patterns. Furthermore, our data showed potentially important changes in areas of metabolism, such as the glyoxylate metabolism, hitherto not suspected to be components of plant defense.