Preoperative Short-term High-Carbohydrate Diet Provides More High-Quality Transplantable Fat and Improves the Outcome of Fat Grafts in Mice
Preoperative Short-term High-Carbohydrate Diet Provides More High-Quality Transplantable Fat and Improves the Outcome of Fat Grafts in Mice
复制标题
术前短期高碳水化合物饮食提供更多高质量的可移植脂肪并改善小鼠脂肪移植的结果
DOI：
10.1093/asj/sjac105
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发表时间：
2022-05-30
影响因子：
2.9
通讯作者：
Yuan，Yi
中科院分区：
文献类型：
--
作者：
Niu，Xingtang;Zhang，Yuchen;Yuan，Yi
背景低BMI的患者可能没有足够的高质量脂肪组织用于移植。高能量饮食对脂肪组织和移植物保留的影响尚不清楚。ObjectivesThe authors discussed the inguinal fat pad alteration in mice fed on a short-time high-fat diet（HFD）or a high-carbonate diet（HCD）preoperative and the morphological and tissue differences after transplantation.（60%千卡来自脂肪，20%来自碳水化合物）、HCD（9.3%千卡来自脂肪，80.1%来自碳水化合物）或正常（12%千卡来自脂肪，67%千卡来自碳水化合物）饮食2或4周。苏木精和伊红染色以及CD 34和增殖细胞核抗原免疫染色后进行组织学分析。免疫印迹法检测解偶联蛋白-1的表达。将各组的脂肪垫移植到受体小鼠的背部区域，并在移植后4、8和12周测定形态学和组织学变化。血管内皮生长因子-α和血小板衍生生长因子-α的表达采用定量聚合酶链反应测定。结果腹股沟脂肪垫体积增加的HFD和HCD组。喂食HCD的小鼠腹股沟脂肪中存在多室脂肪细胞，结合解偶联蛋白-1含量的增加，提示脂肪细胞布朗宁。HCD移植物表现出较高的体积保留和减少油囊肿的形成，可能归因于更好的血管生成和adipogenesis.ConclusionsHCD扩大脂肪组织和提高移植物存活率，这可能是由于布朗宁的脂肪移植前和移植后血管生成增强。
BackgroundPatients with a low BMI may have inadequate high-quality adipose tissue for transplantation. The influence of high-energy diets on adipose tissue and graft retention remains unknown.ObjectivesThe authors explored inguinal fat pad alternation in mice fed on a short-time high-fat diet (HFD) or a high-carbohydrate diet (HCD) preoperatively and the morphological and histological differences after transplantation.MethodsMice were fed HFD (60% kcal from fat, 20% from carbohydrate), HCD (9.3% kcal from fat, 80.1% from carbohydrate), or normal (12% kcal from fat, 67% kcal from carbohydrate) diets for 2 or 4 weeks. Histological analyses were carried out following hematoxylin and eosin staining as well as CD34 and proliferating cell nuclear antigen immunostaining. The uncoupling protein-1 expression was determined by western blotting. Fat pads from each group were grafted into the dorsal region of the recipient mice, and morphological and histological changes were determined 4, 8, and 12 weeks posttransplantation. Vascular endothelial growth factor-α and platelet-derived growth factor-α expression were determined using quantitative polymerase chain reaction.ResultsThe inguinal fat pad volume increased in the HFD and HCD groups. The presence of multilocular adipocytes in inguinal fat of HCD-fed mice, combined with the increased uncoupling protein-1 content, suggested adipocyte browning. HCD grafts showed higher volume retention and reduced oil cyst formation, possibly attributed to better angiogenesis and adipogenesis.ConclusionsHCD enlarged adipose tissue and improved graft survival rates, which may be due to the browning of fat before grafting and enhanced angiogenesis after grafting.