Short interfering RNA directed against the E2F-1 gene suppressing gastric cancer progression in vitro
Short interfering RNA directed against the E2F-1 gene suppressing gastric cancer progression in vitro
复制标题
针对E2F-1基因的短干扰RNA在体外抑制胃癌进展
DOI：
10.3892/or_00000360
复制
发表时间：
2009-05-01
期刊：
影响因子：
4.2
通讯作者：
Xiao, Qiang
中科院分区：
文献类型：
--
作者：
Xie, Yubo;Yin, Yongshuo;Xiao, Qiang
胃癌是中国第三大常见癌症。E2 F-1的持续过表达是胃癌的特征性特征。RNA干扰（RNA interference，RNAi）是一种抑制基因表达的有效手段，为胃癌的治疗提供了一条新的途径。本研究构建了Psilencer 4.1-E2 F-1 siRNA重组质粒，并将其转染胃癌MGC-803细胞。我们的数据表明，E2 F-1 siRNA导致内源性E2 F-1 mRNA和蛋白表达的抑制，通过实时定量RT-PCR和Western印迹测定。此外，同时沉默E2 F-1导致肿瘤细胞增殖活性降低和凋亡细胞百分比升高。体外实验观察其对肿瘤细胞迁移和侵袭能力的抑制作用。综上所述，靶向E2 F-1的siRNA可以有效地抑制胃癌的进展，并可能用作有效的治疗。
Gastric cancer is the third most common cancer in China. The sustained overexpression of E2F-1 is a characteristic feature of gastric cancer. RNA interference (RNAi), which has been proven to be a powerful tool for suppressing gene expression, may provide a promising way forward in gastric cancer therapy. In this study, we constructed the recombinant Psilencer 4.1- E2F-1 siRNA plasmids and transfected them into gastric cancer MGC-803 cells in vitro. Our data demonstrated that E2F-1 siRNA led to inhibition of endogenous E2F-1 mRNA and protein expression as determined by real-time quantitative RT-PCR and Western blotting. Furthermore, simultaneous silencing of E2F-1 resulted in a reduction of tumor cell proliferation activity and a higher percentage of apoptotic cells. The inhibition of migration and invasion potential of tumor cells was investigated in vitro. In summary, siRNA targeting of E2F-1 can effectively inhibit gastric cancer progression and may be used as a potent therapy.