Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of cross-kingdom regulation by microRNA
Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of cross-kingdom regulation by microRNA
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外源植物 MIR168a 特异性靶向哺乳动物 LDLRAP1：microRNA 跨界调节的证据
DOI：
10.1038/cr.2011.158
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发表时间：
2012-01-01
期刊：
影响因子：
44.1
通讯作者：
Zhang, Chen-Yu
中科院分区：
文献类型：
--
作者：
Zhang, Lin;Hou, Dongxia;Zhang, Chen-Yu
我们之前的研究表明，哺乳动物血清和血浆中稳定的microRNAs （miRNAs）从组织和细胞中积极分泌，可以作为一类新的疾病生物标志物，并在细胞间通讯中充当信号分子。在这里，我们报告了一个令人惊讶的发现，即外源植物miRNAs存在于各种动物的血清和组织中，这些外源植物miRNAs主要是通过食物摄入口服获得的。MIR168a在水稻中含量丰富，是中国人血清中含量最高的外源植物mirna之一。体外和体内功能研究表明，MIR168a可以结合人/小鼠低密度脂蛋白受体适配蛋白1 (LDLRAP1) mRNA，抑制肝脏中LDLRAP1的表达，从而减少小鼠血浆中LDL的去除。这些发现表明，食物中的外源植物mirna可以调节哺乳动物靶基因的表达。
Our previous studies have demonstrated that stable microRNAs (miRNAs) in mammalian serum and plasma are actively secreted from tissues and cells and can serve as a novel class of biomarkers for diseases, and act as signaling molecules in intercellular communication. Here, we report the surprising finding that exogenous plant miRNAs are present in the sera and tissues of various animals and that these exogenous plant miRNAs are primarily acquired orally, through food intake. MIR168a is abundant in rice and is one of the most highly enriched exogenous plant miRNAs in the sera of Chinese subjects. Functional studies in vitro and in vivo demonstrated that MIR168a could bind to the human/mouse low-density lipoprotein receptor adapter protein 1 (LDLRAP1) mRNA, inhibit LDLRAP1 expression in liver, and consequently decrease LDL removal from mouse plasma. These findings demonstrate that exogenous plant miRNAs in food can regulate the expression of target genes in mammals.