Selective recognition of oncogene promoter G-quadruplexes by Mg2+.
Selective recognition of oncogene promoter G-quadruplexes by Mg2+.
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DOI：
10.1016/j.bbrc.2010.10.065
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发表时间：
2010-11
影响因子：
3.1
通讯作者：
Yi-yong Yan;Jing Lin;Tian-Miao Ou;Jia-Heng Tan;Ding Li;L. Gu;Zhishu Huang
中科院分区：
文献类型：
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作者：
Yi-yong Yan;Jing Lin;Tian-Miao Ou;Jia-Heng Tan;Ding Li;L. Gu;Zhishu Huang
Mg2+is one of the most important cations in cells, affecting the structures and functions of the proteins and nucleic acids. It should be noted that Mg2+is indispensable in DNA transcription, where G-quadruplex is believed to be actively involved. Therefore, it is important to investigate the influence of Mg2+on G-quadruplex. Here we studied the effect of Mg2+on G-quadruplex DNA with CD, FRET, EMSA, and PCR-stop assay. We found that various G-quadruplexes could be differentiated through simultaneous addition of both K+and Mg2+, which could be used for selective identification of G-quadruplexes in promoter oncogene but not in telomere. Mg2+at physiological relevant concentration not only greatly enhanced the thermostability of oncogene G-quadruplexes but also efficiently protected them from unfolding by their complementary strands, which revealed the great impact of Mg2+on the equilibrium between promoter G-quadruplex and duplex DNA. The PCR-stop assay further confirmed that Mg2+could affect gene transcription by stabilizing promoter G-quadruplex. The above studies were carried out for various G-quadruplexes of varying sequences in promoter oncogenes and telomeric region. Our results suggest that Mg2+may be a key regulator for G-quadruplexes of oncogene promoter, which can subsequently affect the expression of related genes.
