A testis-specific and testis developmentally regulated tumor protein D52 (TPD52)-like protein TPD52L3/hD55 interacts with TPD52 family proteins
A testis-specific and testis developmentally regulated tumor protein D52 (TPD52)-like protein TPD52L3/hD55 interacts with TPD52 family proteins
复制标题
DOI：
10.1016/j.bbrc.2006.03.208
复制
发表时间：
2006-06-09
影响因子：
3.1
通讯作者：
Li, Jianmin
中科院分区：
文献类型：
--
作者：
Cao, Qinhong;Chen, Jie;Li, Jianmin
肿瘤蛋白D52样蛋白（TPD 52）是一种携带小卷曲螺旋基序的蛋白质，首先在乳腺癌中发现。TPD 52及其相关蛋白参与细胞增殖、凋亡和囊泡运输。迄今为止，已经鉴定了三个人TPD 52成员，命名为hD 52（TPD 52）、hD 53（TPD 52 L1）和hD 54（TPD 52 L2）。该蛋白家族最重要的特征是高度保守的卷曲螺旋基序，其是与其他TPD 52样蛋白的同源和异源相互作用所必需的。在此，我们发现了一个新的TPD 52样序列（TPD 52 L3，或hD 55）在人类睾丸cDNA微阵列。序列分析表明，hD 55含有卷曲螺旋结构，与其它TPD 52样序列相比高度保守。酵母双杂交和GST pull-down分析表明hD 55与hD 52、hD 53、hD 54及其自身相互作用。cDNA微阵列检测发现hD 55在成人睾丸中的表达水平是胎儿睾丸的5.6倍。此外，表达谱显示hD 55是睾丸特异性的，表明hD 55在睾丸发育和精子发生中的潜在作用。(c)2006年爱思唯尔公司All rights reserved.
Tumor protein D52-like proteins (TPD52) are small coiled-coil motif bearing proteins that were first identified in breast cancer. TPD52 and related proteins have been implicated in cell proliferation, apoptosis, and vesicle trafficking. To date, three human TPD52 members had been identified, named hD52 (TPD52), hD53 (TPD52L1), and hD54 (TPD52L2). The most important characteristic of the protein family is a highly conserved coiled-coil motif that is required for homo- and heteromeric interaction with other TPD52-like proteins. Herein, we identified a novel TPD52-like sequence (TPD52L3, or hD55) in human testis using cDNA microarray. Sequence analysis of the deduced protein suggests that hD55 contains a coiled-coil motif and is highly conserved compared with other TPD52-like sequences. Yeast two-hybrid and GST pull-down assays revealed that hD55 interacts with hD52, hD53, hD54, and itself. cDNA microarray detection found that hD55 was expressed at 5.6-fold higher levels in adult testis than in fetal testis. Additionally, the expression profile shows that hD55 is testis-specific, indicating a potential role for hD55 in testis development and spermatogenesis. (c) 2006 Elsevier Inc. All rights reserved.