HBV X protein targets HIV tat-binding protein 1
HBV X protein targets HIV tat-binding protein 1
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DOI：
10.1006/viro.2001.0883
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发表时间：
2001-04-25
期刊：
影响因子：
3.7
通讯作者：
Shaul, Y
中科院分区：
文献类型：
--
作者：
Barak, O;Aronheim, A;Shaul, Y
HBV X蛋白（HBx）与肝细胞癌的发生、发展密切相关。HBx对细胞具有多效性效应，表明病毒-宿主细胞相互作用中存在多个靶点。我们采用基于细胞质的双杂交筛选，并确定了HIV Tat结合蛋白1（Tbp 1）作为一种新的HBx相互作用蛋白。Tbp 1在酵母和动物细胞中与HBx在体内相互作用。这种相互作用映射到Tbp 1功能重要的ATP结合基序。此外，HBx和Tbp 1相互作用在功能上是重要的，并调节HBV转录。Tbpl同源物，如Sug 1，是蛋白酶体19 S调节帽颗粒的已知成员，并且也涉及转录共激活。值得注意的是，Tbp 1和Sug 1与多种病毒效应蛋白相互作用，包括HIV达特、SV 40大T抗原和腺病毒E1 A，确立了这些蛋白作为病毒致癌基因的重要靶标。(C)北京：科学出版社.
The HBV X protein (HBx) is implicated in infection and development of hepatocellular carcinoma. HBx has a pleiotropic effect on cells, suggesting multiple targets in the virus-host cell interaction. We employed the cytoplasmic-based two-hybrid screen and identified the HIV Tat-binding protein 1 (Tbp1) as a novel HBx interacting protein. Tbp1 interacts in vivo with HBx both in yeast and in animal cells. This interaction maps to the functionally important ATP-binding motif of Tbp1. Furthermore, HBx and Tbp1 interaction is functionally significant and regulates HBV transcription. Tbpl homologues, such as Sug1, are known members of the proteasome 19S regulatory cap particle and have also been implicated in transcription coactivation. Remarkably, Tbp1 and Sug1 interact with multiple viral effector proteins including HIV Tat, SV40 large T antigen, and adenovirus E1A, establishing these proteins as important targets of the viral oncogenes. (C) 2001 Academic Press.