Effect of carotenoid on calcium content and alkaline phosphatase activity in rat femoral tissues in vitro: the unique anabolic effect of beta-cryptoxanthin.
Effect of carotenoid on calcium content and alkaline phosphatase activity in rat femoral tissues in vitro: the unique anabolic effect of beta-cryptoxanthin.
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类胡萝卜素对体外大鼠股骨组织钙含量和碱性磷酸酶活性的影响：β-隐黄质独特的合成代谢作用。
DOI：
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发表时间：
2003
影响因子：
2
通讯作者：
S. Uchiyama
中科院分区：
文献类型：
--
作者：
M. Yamaguchi;S. Uchiyama
本文研究了不同种类的类胡萝卜素和芦丁对离体幼鼠股骨干和股骨干骺端组织钙含量和碱性磷酸酶活性的影响。将骨组织在含有载体或化合物（10（-8）-10（-6）M）的无血清Dulbecco改良Eagle培养基中培养48小时。β-隐黄素（10（-7）或10（-6）M）的存在导致股骨骨干和股骨干骺端组织中钙含量和碱性磷酸酶活性显著增加。此外，使用的最低浓度（10（-8）M）的β-隐黄素导致骨干和干骺端钙含量显著增加。叶黄素（10（-8）-10（-6）M）对骨干和干骺端钙含量及骨干碱性磷酸酶活性无影响，而10（-7）和10（-6）M叶黄素显著降低干骺端碱性磷酸酶活性。番茄红素（10（-8）-10（-6）M）或芦丁（10（-8）-10（-6）M）对骨钙含量和碱性磷酸酶活性没有显著影响。本研究表明，类胡萝卜素β-隐黄素在体外对骨钙化具有独特的合成代谢作用。
The effects of various carotenoids and rutin on calcium content and alkaline phosphatase activity in the femoral-diaphyseal and femoral-metaphyseal tissues of young rats in vitro were investigated. Bone tissues were cultured for 48 h in serum-free Dulbecco's modified Eagle's medium containing either vehicle or a compound (10(-8)-10(-6) M). The presence of beta-cryptoxanthin (10(-7) or 10(-6) M) caused a significant increase in calcium content and alkaline phosphatase activity in the femoral-diaphyseal and femoral-metaphyseal tissues. Also, the lowest concentration (10(-8) M) of beta-cryptoxanthin used caused a significant increase in diaphyseal and metaphyseal calcium content. Lutein (10(-8)-10(-6) M) had no effect on diaphyseal and metaphyseal calcium contents and diaphyseal alkaline phosphatase activity, while 10(-7) and 10(-6) M lutein significantly decreased metaphyseal alkaline phosphatase activity. Lycopene (10(-8)-10(-6) M) or rutin (10(-8)-10(-6) M) did not have a significant effect on bone calcium content and alkaline phosphatase activity. The present study suggests that the carotenoid beta-cryptoxanthin has a unique anabolic effect on bone calcification in vitro.
血浆类胡萝卜素作为蔬菜摄入量的生物标志物：明尼苏达大学癌症预防研究单位喂养研究。
DOI：
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发表时间：
1995
期刊：
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
影响因子：
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作者：
Martini,MC;Campbell,DR;Gross,MD;Grandits,GA;Potter,JD;Slavin,JL
通讯作者：
Slavin,JL