A new member of the I kappa B protein family, I kappa B epsilon, inhibits RelA (p65)-mediated NF-kappa B transcription
A new member of the I kappa B protein family, I kappa B epsilon, inhibits RelA (p65)-mediated NF-kappa B transcription
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DOI：
10.1128/mcb.17.10.6184
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发表时间：
1997-10-01
影响因子：
5.3
通讯作者：
Nabel, GJ
中科院分区：
文献类型：
--
作者：
Li, Z;Nabel, GJ
I κ B家族的一个新成员在酵母双杂交筛选中被鉴定为与NF-κ B的p50亚基相关的蛋白质。与先前已知的I κ B蛋白类似，该成员I κ B具有连续的锚蛋白重复序列。I κ B受体mRNA在人体不同组织中广泛表达，以脾脏表达量最高。在体外和体内，I κ B与不同的NF-κ B蛋白，包括p65（RelA）、c-Rel、p50和p52相互作用，并有效地抑制p50-p65和p50-c-Rel复合物的DNA结合活性。I κ B mRNA抑制（RelA依赖性）转录激活，I κ B mRNA在特定细胞类型中以不同水平表达，并在转化的B细胞系中组成型合成，与非B细胞系中的I κ B α相比，它还显示对肿瘤坏死因子α、白细胞介素-1或佛波酯刺激的不同诱导。因此，I kappa B是一个新的I kappa B家族成员，它为NF-κ B依赖性转录的调节提供了另一种机制。
A novel member of the I kappa B family has been identified as a protein that associated with the p50 subunit of NF-kappa B in a yeast two-hybrid screen, Similar to previously known I kappa B proteins, this member, I kappa B epsilon, has sis consecutive ankyrin repeats. I kappa B epsilon mRNA is widely expressed in different human tissues, with highest le,els in spleen. testis, and lung, I kappa B epsilon interacts with different NF-kappa B proteins, including p65 (RelA), c-Rel, p50, and p52, in vitro and in vivo and inhibits the DNA-binding activity of both p50-p65 and p50-c-Rel complexes effectively, Endogenous and transfected NF-kappa B (RelA-dependent) transcriptional activation is inhibited by I kappa B epsilon, I kappa B epsilon mRNA is expressed at different levels in specific cell types and is synthesized constitutively in transformed B-cell Lines, It also displays differential induction in response to tumor necrosis factor alpha, interleukin-1, or phorbol ester stimulation compared to I kappa B alpha in non-B cell lines. Therefore, I kappa B epsilon represents a novel I kappa B family member which provides an alternative mechanism for regulation of NF-kappa B-dependent transcription.