Determination of Ochratoxin A with a DNA Aptamer
Determination of Ochratoxin A with a DNA Aptamer
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DOI：
10.1021/jf801957h
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发表时间：
2008-11-26
影响因子：
6.1
通讯作者：
Penner, Gregory
中科院分区：
文献类型：
--
作者：
Cruz-Aguado, Jorge A.;Penner, Gregory
本文描述了一种与赭曲霉毒素A（一种存在于小麦和其他食品中的真菌毒素）具有高亲和力和特异性结合的适配体的鉴定，以及一种基于该适配体的赭曲霉毒素A的定量检测方法。适配体是在体外选择的单链寡核苷酸，用于结合分子靶标。在这项工作中选择的适体显示出在纳摩尔范围内的解离常数，并且不结合结构类似于OTA的化合物，如n -乙酰苯丙氨酸或华法林。所选适配体对赭曲霉毒素b的亲和力降低100倍，可用于天然污染小麦样品中OTA的ppb含量测定。随着传感器、亲和柱和其他分析系统的发展，进一步的工作正在进行中，以扩大这里展示的应用，用于现场和实验室测定食品和农产品中的这种毒素。
This work describes the identification of an aptamer that binds with high affinity and specificity to ochratoxin A (OTA), a mycotoxin that occurs in wheat and other foodstuffs, and a quantitative detection method for OTA based on the use of this aptamer. Aptamers are single-stranded oligonucleotides selected in vitro to bind to molecular targets. The aptamer selected in this work exhibited a dissociation constant in the nanomolar range and did not bind compounds with structures similar to OTA such as N-acetylphenylalanine or warfarin. The aptamer bound with a 100-fold less affinity to ochratoxin B. The selected aptamers could be used for the determination of ppb quantities of OTA in naturally contaminated wheat samples. Further work is ongoing to broaden the application demonstrated here with the development of sensors, affinity columns, and other analytical systems for field and laboratory determination of this toxin in food and agricultural products.