Hydrogel-Immobilized Supercharged Proteins
Hydrogel-Immobilized Supercharged Proteins
复制标题
DOI：
10.1002/adbi.201700240
复制
发表时间：
2018-07-01
影响因子：
4.1
通讯作者：
Jackson, Colin J.
中科院分区：
文献类型：
--
作者：
Campbell, Eleanor C.;Grant, Jacob;Jackson, Colin J.
酶在化学合成、环境生物修复和医学治疗方面的显著催化潜力受到其寿命和稳定性的限制。将酶固定在固体载体上已被证明可以提高生物催化剂的稳定性，但通常依赖于多个化学步骤进行共价附着，并且受各种载体的物理性质的限制。在这里，生产的酶：水凝胶复合物是通过工程描述一个阳离子增压磷酸三酯酶。这些酶：水凝胶复合物在使用80天后没有显示出催化活性的损失，并且当催化剂负载低至0.0008 mol%时，在流动反应器中使用时高达10(5)次周转。此外，观察到对有机溶剂的特殊弹性。酶：水凝胶复合物的使用可能在多种应用中具有价值，例如对映选择性连续流化学，毒素解毒和基化生物材料的形成。
The remarkable catalytic potential of enzymes in chemical synthesis, environmental bioremediation, and medical therapeutics is limited by their longevity and stability. Immobilization of enzymes on solid supports is demonstrated to improve the stability of biocatalysts but often relies on multiple chemical steps for covalent attachment and is limited by the physical properties of the various supports. Here, production of enzyme: hydrogel complexes is described via engineering of a cationic supercharged phosphotriesterase. These enzyme: hydrogel complexes are remarkably robust displaying no loss of catalytic activity after 80 d of use and up to 10(5) turnovers when used in a flow reactor at catalyst loadings as low as 0.0008 mol%. In addition, exceptional resilience to organic solvents is observed. The use of enzyme: hydrogel complexes is likely to be of value in a diverse range of applications such as enantioselective continuous-flow chemistry, detoxification of poisons, and the formation of fundionalized biomaterials.