Microencapsulation of Probiotics by Calcium Alginate-gelatinized Starch with Chitosan Coating and Evaluation of Survival in Simulated Human Gastro-intestinal Condition
Microencapsulation of Probiotics by Calcium Alginate-gelatinized Starch with Chitosan Coating and Evaluation of Survival in Simulated Human Gastro-intestinal Condition
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DOI：
10.22037/ijpr.2014.1550
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发表时间：
2014-07
期刊：
影响因子：
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通讯作者：
Mohammad Ali Khosravi Zanjani;Babak Ghiassi Tarzi;A. Sharifan;N. Mohammadi
中科院分区：
文献类型：
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作者：
Mohammad Ali Khosravi Zanjani;Babak Ghiassi Tarzi;A. Sharifan;N. Mohammadi
微囊化作为最现代的方法之一，对益生菌的存活具有相当大的影响。在这项研究中，干酪乳杆菌 (ATCC 39392) 和两歧双歧杆菌 (ATCC 29521) 采用海藻酸钙糊化淀粉、壳聚糖涂层和菊粉，通过乳化技术进行封装，并在模拟胃液（连同胃蛋白酶，pH=1.5）和模拟肠液（连同胰酶和胆汁盐，pH=8）中在 37 ℃ 下孵育 2 小时。摄氏度。通过扫描电子显微镜和光学显微镜测量微胶囊的形态和尺寸。结果表明，微囊益生菌在模拟胃肠道条件下的存活率显着增加（P < 0.05）。壳聚糖涂层对模拟胃肠道条件下的益生菌起到了显着的保护作用，并且随着壳聚糖涂层的添加，微胶囊的直径增大。总的来说，这项研究表明，壳聚糖包覆的海藻胶糊化淀粉微胶囊可以成功且显着地保护益生菌免受模拟人体胃肠道条件下的不利条件的影响。
Microencapsulation as one of the most modern methods has considerable effects on probiotic survival. In this study Lactobacillus casei (ATCC 39392) and Bifidobacterium bifidum (ATCC 29521) were encapsulated using calcium alginate-gelatinized starch, chitosan coating and inulin via emulsion technique, and were incubated in simulated gastric juice (along with pepsin, pH=1.5) and simulated intestinal juice (along with pancreatin and bile salts, pH = 8) for 2 hours at 37 oC. The morphology and size of microcapsules were measured by scanning electron and optical microscopy. The results indicated that the survival of microencapsulated probiotic increased significantly in simulated gastro-intestinal condition (P < 0.05). Chitosan coating played a significant role in the protection of probiotic bacteria in simulated gastro-intestinal condition and the diameter of the microcapsules increased with the addition of chitosan coating. In general, this study indicated that microencapsulation with alginate-gelatinized starch coated with chitosan could successfully and significantly protect probiotic bacteria against adverse condition of simulated human gastro-intestinal condition.