Functional characterization of chimpanzee cytomegalovirus chemokine, vCXCL-1(CCMV).
Functional characterization of chimpanzee cytomegalovirus chemokine, vCXCL-1(CCMV).
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黑猩猩巨细胞病毒趋化因子 vCXCL-1(CCMV) 的功能特征。
DOI：
10.1016/j.virol.2007.03.002
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发表时间：
2007
期刊：
影响因子：
3.7
通讯作者：
Sparer,TimE
中科院分区：
文献类型：
--
作者：
Miller-Kittrell,Mindy;Sai,Jiqing;Penfold,Mark;Richmond,Ann;Sparer,TimE
人巨细胞病毒（Human cytomegalovirus，HCMV）是免疫功能低下患者和新生儿的重要病原体。HCMV Toledo（Tol）株的病毒趋化因子vCXCL-1与HCMV毒力有关。黑猩猩CMV（CCMV）具有与vCXCL-1 Tolgene UL 146相似的几个基因。为了测试CCMV病毒趋化因子vCXCL-1CCMV是否与vCXCL-1 Tol相似，我们在体外表征了其功能。比较了vCXCL-1 Tol和vCXCL-1CCMV与宿主趋化因子之间的受体结合、活化、趋化性、信号传导和中性粒细胞凋亡。虽然同源物具有相似的活化潜力、趋化特性和信号传导，但vCXCL-1CCMV对CXCR 2的亲和力低70倍，并且在整合素上调和中性粒细胞凋亡方面表现出差异。这些数据表明，尽管vCXCL-1中存在广泛的氨基酸变异性，但CCMV可提供一种模型，用于评估vCXCL-1在体内CMV发病机制中的作用。
Human cytomegaloviruses (HCMVs) are important pathogens in immunocompromised patients and newborns. The viral chemokine, vCXCL-1, of the Toledo (Tol) strain of HCMV has been implicated in HCMV virulence. Chimpanzee CMV (CCMV) has several genes with similarity to the vCXCL-1Tolgene, UL146. In order to test whether the CCMV viral chemokine, vCXCL-1CCMV, is similar to vCXCL-1Tol, we characterized its function in vitro. Receptor binding, activation, chemotaxis, signaling, and apoptosis in neutrophils were compared between vCXCL-1Toland vCXCL-1CCMVand host chemokines. Although the homologues had similar activation potentials, chemotactic properties, and signaling, vCXCL-1CCMVhad a ∼70-fold lower affinity for CXCR2 and displayed differences in integrin upregulation and neutrophil apoptosis. These data demonstrate that in spite of extensive amino acid variability in vCXCL-1, CCMV may provide a model for assessing the role of vCXCL-1 in CMV pathogenesis in vivo.