Ultrasonic separations in analytical biotechnology
Ultrasonic separations in analytical biotechnology
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DOI：
10.1016/s0167-7799(97)01122-0
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发表时间：
1997-12-01
影响因子：
17.3
通讯作者：
Coakley, WT
中科院分区：
文献类型：
--
作者：
Coakley, WT
细胞在兆赫频率noncavitating超声驻波集中在亚毫米的距离，并作为大团块，很容易从悬浮液中的流动或批处理系统。用于灌注杂交瘤培养的超声波过滤器现在可用，来自小体积原型分析规模系统的结果可以为有效的过滤器或批量澄清系统的设计提供信息，用于广泛的细胞大小，浓度和样品体积。在驻波中，水相两相分离的速率以及分析型免疫包被颗粒凝集试验的速率和灵敏度大幅增加。超声操作进行了简要比较免疫磁性和介电泳分离。
Cells in megahertz-frequency noncavitating ultrasonic standing waves concentrate at submillimetre distances and are, as large clumps, easily removed from suspension in flow or batch systems. An ultrasonic filter for perfusion hybridoma culture is now available, Results from small-volume prototype analytical-scale systems can inform the design of effective filter or batch-clarification systems for a wide range of cell sizes, concentrations and sample volumes. Large increases in the rates of aqueous biphasic separations and of the rates and sensitivities of analytical immunocoated particle-agglutination assays occur in standing waves. Ultrasonic manipulation is briefly compared with immunomagnetic and dielectrophoretic separations.