CELLULAR-DISTRIBUTION OF TRANSFERRIN, FERRITIN, AND IRON IN NORMAL AND AGED HUMAN BRAINS
CELLULAR-DISTRIBUTION OF TRANSFERRIN, FERRITIN, AND IRON IN NORMAL AND AGED HUMAN BRAINS
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DOI：
10.1002/jnr.490270421
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发表时间：
1990-12-01
影响因子：
4.2
通讯作者：
MUFSON, EJ
中科院分区：
文献类型：
--
作者：
CONNOR, JR;MENZIES, SL;MUFSON, EJ
对正常人和老年人的许多脑区中的铁转运蛋白、转铁蛋白和铁储存蛋白铁蛋白以及铁进行了免疫组织化学检查。 研究人员对两个年龄组进行了检查：中年组（28-49 岁）和老年组（60-90 岁）。 转铁蛋白、铁蛋白和铁遍布所有检查的大脑区域，主要存在于小而圆形的细胞的核周细胞质中，符合少突胶质细胞的描述。 这些细胞存在于两个年龄段的视神经以及大脑皮层、小脑和嗅球的灰质和白质中。 铁蛋白也存在于大部分大脑区域灰质的小胶质细胞中。 在检查的皮层下区域（纹状体、海马、杏仁核）中，除了少突胶质细胞外，还经常观察到星形胶质细胞含有转铁蛋白、铁蛋白和铁。 细胞标记存在与年龄相关的变化：在最年长的年龄组中，灰质和白质中的星形胶质细胞都含有转铁蛋白，而在年轻组中，皮层下转铁蛋白免疫反应性主要局限于少突胶质细胞。 皮层下大脑区域中的铁蛋白也存在于星形胶质细胞中，但主要局限于灰质中的铁蛋白，即使在最年长的年龄组中也是如此。 铁主要存在于少突胶质细胞中，尽管可以识别出一些铁阳性星形胶质细胞和小胶质细胞。 这些结果表明（1）正常少突胶质细胞含有大脑中发现的大量铁和铁结合蛋白； (2)年龄的增加与铁结合蛋白的细胞分布改变有关，但这种改变是神经胶质细胞特有的。 这些结果表明神经胶质细胞可能具有以前未描述的与金属调节和隔离相关的功能。
The iron transport protein, transferrin, and the iron storage protein ferritin were examined immunohistochemically along with iron in a number of brain regions from normal and aged humans. Two age groups were examined: a middle-aged group (28-49 years), and an older group (60-90 years). Transferrin, ferritin, and iron are found throughout all brain regions examined, predominantly in the perikaryal cytoplasm of cells that are small and round, fitting the description of oligodendrocytes. These cells are present in the optic nerve and in both the gray and white matter of the cerebral cortex, cerebellum, and olfactory bulb in both age groups. Ferritin is also found in microglial cells in the gray matter of most of these brain regions. In the subcortical regions examined (corpus striatum, hippocampus, amygdala), in addition to oligodendrocytes, astrocytes can frequently be observed that contain transferrin, ferritin, and iron. There is an age-related alteration in cell labeling: astrocytes in both gray and white matter contained transferrin in the oldest age group, whereas in the younger group the subcortical transferrin immunoreactivity was confined mostly to oligodendrocytes. Ferritin in the subcortical brain regions is also present in astrocytes but is primarily confined to those in the gray matter, even in the oldest age group. Iron is found predominantly in oligodendrocytes, although a few iron-positive astrocytes and microglia can be identified. These results indicate that (1) normally oligodendrocytes contain much of the iron and iron-binding proteins found in the brain; and (2) an increase in age is associated with altered cellular distribution of iron-binding proteins, but the altered distribution is specific to glial cells. These results suggest glial cells may have previously undescribed functions related to metal regulation and sequestration.