Expressed sequence tag analysis of the bradyzoite stage of Toxoplasma gondii:: Identification of developmentally regulated genes
Expressed sequence tag analysis of the bradyzoite stage of Toxoplasma gondii:: Identification of developmentally regulated genes
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DOI：
10.1128/iai.66.4.1632-1637.1998
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发表时间：
1998-04-01
影响因子：
3.1
通讯作者：
Boothroyd, JC
中科院分区：
文献类型：
--
作者：
Manger, ID;Hehl, A;Boothroyd, JC
刚地弓形虫是一种原生动物寄生虫，在人类和动物中引起广泛感染。在这种寄生虫的生活史中产生两种主要的无性形式：快速分裂的速殖子和分裂较慢的包囊缓殖子。为了进一步研究这两种形式之间的差异，我们已经产生了大量的表达序列标签（EST）从两个无性阶段。以前，我们从速殖子中获得了类似于7，400个EST的数据（J. Ajioka et al.，Genome Res.8：18-28，1998），在这里，我们报告了从感染小鼠的包囊中纯化的缓殖子的近似2，500个EST的分析结果。我们还报道了760个体外诱导速殖子分化为缓殖子的寄生虫EST的分析结果。从缓殖子和速殖子的数据集的比较揭示了许多以前未表征的序列簇，这是很大程度上或完全特定于一个或其他发展阶段。这个类别包括一种缓殖子特异性的烯醇化酶。结合先前鉴定的缓殖子特异性形式的乳酸脱氢酶，这一发现表明在此阶段通过糖酵解途径下端的通量存在显著差异。因此，该数据集的产生提供了有价值的见解的代谢和生长的寄生虫在包囊的形式，并代表了大量的信息，为进一步研究弓形虫的发展。
Toxoplasma gondii is a protozoan parasite responsible for widespread infections in humans and animals. Two major asexual forms are produced during the life cycle of this parasite: the rapidly dividing tachyzoite and the more slowly dividing, encysted bradyzoite. To further study the differentiation between these two forms, we have generated a large number of expressed sequence tags (ESTs) from both asexual stages. Previously, we obtained data on similar to 7,400 ESTs from tachyzoites (J. Ajioka et al., Genome Res. 8:18-28, 1998), Here, we report the results from analysis of similar to 2,500 ESTs from bradyzoites purified from the cysts of infected mice. We also report the results from analysis of 760 ESTs from parasites induced to differentiate from tachyzoites to bradyzoites in vitro. Comparison of the data sets from bradyzoites and tachyzoites reveals many previously uncharacterized sequence clusters which are largely or completely specific to one or other developmental stage. This class includes a bradyzoite-specific form of enolase. Combined with the previously identified bradyzoite-specific form of lactate dehydrogenase, this finding suggests significant differences in flux through the lower end of the glycolytic pathway in this stage. Thus, the generation of this data set provides valuable insights into the metabolism and growth of the parasite in the encysted form and represents a substantial body of information for further study of development in Toxoplasma.