Evaluation of the epidemiological relevance of variable-number tandem-repeat genotyping of Mycobacterium bovis and comparison of the method with IS6110 restriction fragment length polymorphism analysis and spoligotyping
Evaluation of the epidemiological relevance of variable-number tandem-repeat genotyping of Mycobacterium bovis and comparison of the method with IS6110 restriction fragment length polymorphism analysis and spoligotyping
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DOI：
10.1128/jcm.01775-05
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发表时间：
2006-06-01
影响因子：
9.4
通讯作者：
Fauville-Dufaux, Maryse
中科院分区：
文献类型：
--
作者：
Allix, Caroline;Walravens, Karl;Fauville-Dufaux, Maryse
在许多动物和人类疾病病例中，牛分枝杆菌污染的来源仍不清楚。一个主要的限制是缺乏足够的信息或流行病学良好评价的分子分型方法。在这里，我们报告了基于29个分枝杆菌散布重复单位可变数目串联重复（MIRU-VNTR）基因座对基因型127 M的高通量方法的评价。从77个不同比利时农场的牛中分离的牛病毒，代表了1995年至2003年获得的全国范围内的收集。MIRU-VNTR的稳定性通过分析一系列共74株分离株得到证明，这些分离株来自单个农场或具有已确定流行病学联系的不同农场的不同动物。将基因分型结果和基因型多样性（h）与IS 6110限制性片段长度多态性（RFLP）分析和Spoligotyping进行比较。在68个没有已知流行病学联系的分离株中，MIRU-VNTR分型比RFLP分析或spoligotyping更好地区分分离株单独（32对16和17基因型; h = 0.91对0.73和0.85，分别）或组合（32对28基因型; h = 0.91对0.92）。最大分辨率已经实现了一个子集的9个位点。基于IS 6110 RFLP分析、spoligotyping和MIRU-VNTR标记的遗传关系的一致性与M.牛这些结果支持MIRU-VNTR分型作为分析M种群结构的一种方便且具有区分性的技术。牛更详细地和解决一些仍然悬而未决的问题，在流行病学的病原体。
Sources of Mycobacterium bovis contamination remain unclear for many cases of animal and human disease. A major limitation is the lack of sufficiently informative or epidemiologically well evaluated molecular methods for typing. Here, we report an evaluation of a high-throughput method based on 29 mycobacterial interspersed repetitive unit-variable-number tandem-repeat (MIRU-VNTR) loci to genotype 127 M. bovis isolates from cattle from 77 different Belgian farms, representative of a nationwide collection obtained from 1995 to 2003. MIRU-VNTR stability was demonstrated by analyzing a series of 74 isolates in total, obtained from different animals from a single farm or from different farms with an identified epidemiological link. The genotyping results and the genotypic diversity (h) were compared with those obtained by IS6110 restriction fragment length polymorphism (RFLP) analysis and spoligotyping. Among 68 isolates with no known epidemiological link, MIRU-VNTR typing discriminated better than either RFLP analysis or spoligotyping, with isolates taken individually (32 versus 16 and 17 genotypes; h = 0.91 versus 0.73 and 0.85, respectively) or in combination (32 versus 28 genotypes; h = 0.91 versus 0.92). Maximal resolution was already achieved with a subset of 9 loci. The observed congruence of the genetic relationships based on IS6110 RFLP analysis, spoligotyping, and MIRU-VNTR markers is consistent with a clonal population structure of M. bovis. These results support MIRU-VNTR typing as a convenient and discriminatory technique for analysis of the population structure of M. bovis in much greater detail and for addressing some still unresolved issues in the epidemiology of the pathogen.