Rat dorsal root ganglia express distinctive forms of the alpha2 calcium channel subunit.
Rat dorsal root ganglia express distinctive forms of the alpha2 calcium channel subunit.
复制标题
大鼠背根神经节表达独特形式的 α2 钙通道亚基。
DOI：
10.1097/00001756-200011090-00010
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发表时间：
2000
期刊：
影响因子：
1.7
通讯作者：
Luo,ZD
中科院分区：
文献类型：
--
作者：
Luo,ZD
钙通道α 2 δ亚基是一个糖基化的结构亚基，与α 2亚基和δ肽一致。以往的研究表明α 2亚基在大鼠脊髓和背根神经节（DRG）中有不同的表达。本研究探讨了差异糖基化是否是不同α 2 δ亚基的分子基础。用Western印迹法比较大鼠脊髓、背根神经节、脑和骨骼肌中去糖基化α 2亚基的迁移模式。报告的数据表明，DRG中存在两种形式的α 2亚基，至少在糖基化水平上与检查的其他组织中的α 2亚基不同。因此，翻译后修饰在α 2 δ亚基的组织特异性和功能性表达中可能是重要的。
Calcium channel α 2 δ subunit is a glycosylated structural subunit consistent of the α 2 subunit and the δ peptide. Previous studies have indicated distinctive α 2 subunit expression in rat spinal cord and dorsal root ganglia (DRG). This study examined whether differential glycosylation underlies the molecular basis of distinct α 2 δ subunits. The migration patterns of deglycosylated α 2 subunits from rat spinal cord, DRG, brain and skeletal muscle were compared in Western blots. The data reported indicate that there are two forms of the α 2 subunit in DRG that are different from the α 2 subunit in other tissues examined, at least at the glycosylation level. Thus, post-translational modification may be important in tissue specific and functional expression of the α 2 δ subunit.