Fluorescent nanoparticle beacon for logic gate operation regulated by strand displacement.
Fluorescent nanoparticle beacon for logic gate operation regulated by strand displacement.
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DOI：
10.1021/am401493d
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发表时间：
2013-06
影响因子：
9.5
通讯作者：
J. Yang;Lingjing Shen;Jingjing Ma;H. I. Schlaberg;Shi Liu;Jin Xu;Cheng Zhang
中科院分区：
文献类型：
--
作者：
J. Yang;Lingjing Shen;Jingjing Ma;H. I. Schlaberg;Shi Liu;Jin Xu;Cheng Zhang
提出了一种以DNA/金纳米粒子(AuNP)共轭为基本工作单元，利用荧光信标探头检测输出信号来构建逻辑系统的机制。为了实现逻辑电路，一个自组装的DNA结构被连接到纳米颗粒上，形成荧光信标。此外，在多电平串位移调节的辅助下，实现了级联逻辑门。利用荧光信号、凝胶电泳法和透射电子显微镜对计算结果进行了检测。这项工作有望证明基于荧光纳米粒子信标的级联逻辑系统的可行性，并建议在DNA计算和生物技术中应用。
A mechanism is developed to construct a logic system by employing DNA/gold nanoparticle (AuNP) conjugates as a basic work unit, utilizing a fluorescent beacon probe to detect output signals. To implement the logic circuit, a self-assembly DNA structure is attached onto nanoparticles to form the fluorescent beacon. Moreover, assisted by regulation of multilevel strand displacement, cascaded logic gates are achieved. The computing results are detected by methods using fluorescent signals, gel electrophoresis and transmission electron microscope (TEM). This work is expected to demonstrate the feasibility of the cascaded logic system based on fluorescent nanoparticle beacons, suggesting applications in DNA computation and biotechnology.