Batch-feeding whole-cell catalytic synthesis of -arbutin by amylosucrase from Xanthomonas campestris
Batch-feeding whole-cell catalytic synthesis of -arbutin by amylosucrase from Xanthomonas campestris
复制标题
野油菜黄单胞菌淀粉蔗糖酶分批全细胞催化合成β-熊果苷
DOI：
10.1007/s10295-019-02143-z
复制
发表时间：
2019
影响因子：
3.4
通讯作者：
Chen Xiaolong
中科院分区：
文献类型：
--
作者：
Zhu Linjiang;Jiang Dan;Zhou Yaoyao;Lu Yuele;Fan Yongxian;Chen Xiaolong
α-熊果苷是一种有效的美白化妆品成分，可通过对苯二酚糖基化反应合成。玉米黄单胞菌Xanthomonascampestrispv.campestris8004的淀粉蔗糖酶（Amylsucrase，Amy-1）是一种利用蔗糖的糖基化对苯二酚酶。其动力学参数显示对苯二酚的亲和力比麦芽糖利用α-糖苷酶高7倍。当糖苷供体与受体的摩尔比为80：1时，HQ的糖基化可以快速实现，转化率超过99%。设计了一种分批进料催化方法，以消除HQ抑制，并具有高生产率（> 36.4 mM h-1）。此外，为了消除对苯二酚氧化产物积累所造成的严重抑制，进一步提出了全细胞催化。在15 h内，α-熊果苷的摩尔转化率达到95%。因此，Amy-1的分批补料全细胞生物催化是一种很有前途的生产α-熊果苷的技术，可以提高产率和摩尔转化率。
α-Arbutin is an effective skin-whitening cosmetic ingredient and can be synthesized through hydroquinone glycosylation. In this study, amylosucrase (Amy-1) fromXanthomonas campestrispv.campestris8004 was newly identified as a sucrose-utilizing glycosylating hydroquinone enzyme. Its kinetic parameters showed a seven-time higher affinity to hydroquinone than maltose-utilizing α-glycosidase. The glycosylation of HQ can be quickly achieved with over 99% conversion when a high molar ratio of glycoside donor to acceptor (80:1) was used. A batch-feeding catalysis method was designed to eliminate HQ inhibition with high productivity (> 36.4 mM h−1). Besides, to eliminate the serious inhibition caused by the accumulated hydroquinone oxidation products, the whole-cell catalysis was further proposed. 306 mM of α-arbutin was finally achieved with 95% molar conversion rate within 15 h. Hence, the batch-feeding whole-cell biocatalysis by Amy-1 is a promising technology for α-arbutin production with enhanced yield and molar conversion rate.