CERIUM(IV)-OLIGODNA HYBRID AS HIGHLY SELECTIVE ARTIFICIAL NUCLEASE
CERIUM(IV)-OLIGODNA HYBRID AS HIGHLY SELECTIVE ARTIFICIAL NUCLEASE
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DOI：
10.1080/10610279408029859
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发表时间：
1994-01-01
影响因子：
3.3
通讯作者：
KODAMA, T
中科院分区：
文献类型：
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作者：
KOMIYAMA, M;SHIIBA, T;KODAMA, T
利用亚氨基二乙酸配体将铈（IV）离子连接到19-mer DNA的5'端。在pH 7.2和30℃的条件下，杂交体选择性地在靶位点（底物DNA 19-mer部分的3'侧）切割40-mer的DNA，该DNA具有与杂交体DNA互补的19-mer序列。铈（IV）离子将胸腺苷基（3′-5′）水解为胸腺苷，而没有同时对核糖进行氧化裂解，表明杂化物的选择性裂解是通过磷酸二酯键的水解进行的。
Cerium(IV) ion is attached to the 5'-end of a 19-mer DNA by use of iminodiacetate Ligand. At pH 7.2 and 30 degrees C, the hybrid selectively cuts a 40-mer DNA, which has 19-mer sequence complementary with the DNA in the hybrid, at the target site (the 3'-side of the 19-mer portion of the substrate DNA). Cerium(IV) ion hydrolyzes thymidylyl(3'-5')thymidine to thymidine without concurrent oxidative cleavage of the ribose, indicating that the selective scission by the hybrid proceeds via the hydrolysis of the phosphodiester linkage.