Highly sensitive determination of lanthanides by capillary electrophoresis with direct visible detection after precapillary complexation with aromatic polyaminocarboxylate and additionally applying dynamic ternary complexation with nitrilotriacetic acid
Highly sensitive determination of lanthanides by capillary electrophoresis with direct visible detection after precapillary complexation with aromatic polyaminocarboxylate and additionally applying dynamic ternary complexation with nitrilotriacetic acid
复制标题
与芳香族聚氨基羧酸盐进行毛细管前络合，并另外与次氮基三乙酸进行动态三元络合后，通过毛细管电泳进行高灵敏度测定，并进行直接可见光检测
DOI：
10.1002/elps.200500828
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发表时间：
2006
期刊：
影响因子：
2.9
通讯作者：
S. Hoshi
中科院分区：
文献类型：
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作者：
S. Saito;Naoki Danzaka;S. Hoshi
将一种新合成的芳香族聚氨基羧酸酯（NBD‐ABEDTA，H4 L）作为镧系离子（Ln 3+）的螯合剂应用于毛细管前衍生毛细管电泳。Ln-L复合物由于其甲基-EDTA配位结构而提供解离动力学稳定性，并且由于其硝基苯并呋咱部分而在469 nm的可见光区具有高吸光率（εmax = 2.4×104 cm−1 mol−1 dm 3）。 基于一个独特的概念，将配体用于毛细管区带电泳：在一个中心金属离子上进行毛细管前和动态毛细管络合，以实现高分辨率。作为三元复合物形成剂，将与母复合物（Ln-L）结合的亚氨基二乙酸盐（IDA）加入载体缓冲液中。 在9.5 mmol·dm−3硼酸盐（pH 9.45）和33.5 mmol·dm−3 IDA的载体缓冲溶液中，Ln 3+离子的分离度得到了显著提高。 除Pr-Sm外，各Ln配合物均得到有效分离。此外，由于载体溶液中不存在L，因此基线波动稳定，LOD较低（通常为4.2×10−7 mol·dm−3）。 这强烈表明，Ln-L络合物即使在IDA大量过量的情况下也是动力学稳定的。出乎意料的是，迁移的顺序与原子序数不同，在Nd处反转。这是由于剩余配位的空穴大小对三元络合和Ln 3+的电子密度的影响。
A newly synthesized aromatic polyaminocarboxylate (NBD‐ABEDTA, H4L) was applied to precapillary derivatizing capillary electrophoresis as a chelating reagent for lanthanide ions (Ln3+). The Ln‐L complexes provide both kinetic stability on dissociation due to their methyl‐EDTA coordinating structure, and high light absorptivity (εmax = 2.4×104 cm−1 mol−1 dm3) in the visible region at 469 nm thanks to their nitrobenzofurazan moiety. A ligand was employed for capillary zone electrophoresis based on a unique concept: both precapillary and dynamic on‐capillary complexation were carried out on one center‐metal ion to achieve high resolution. As a ternary complex‐formation agent, iminodiacetate (IDA), bound to the mother complex (Ln‐L), was added to the carrier buffer solution. The carrier buffer solution of 9.5 mmol·dm−3 (pH 9.45) borate and 33.5 mmol·dm−3 IDA, drastically improved the resolution among Ln3+ ions. Each of the Ln complexes was effectively separated, except for Pr‐Sm. Furthermore, the absence of L from the carrier solution, which stabilizes the baseline fluctuation, provided low LOD (typically 4.2×10−7 mol·dm−3). This strongly suggests that Ln‐L complexes are kinetically stable even with a large excess of IDA. Quite unexpectedly, the order of migration differs from that of the atomic number, inverting at Nd. This is due to the effect of the cavity size of the residual coordination sites on the ternary complexation and the electronic density of Ln3+.
利用碳酸根离子增强分辨率，通过毛细管电泳高选择性同时检测作为动力学稳定的芳香族聚氨基羧基复合物的镧系元素 (III) 离子
DOI：
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发表时间：
2004
期刊：
Analytical and Bioanalytical Chemistry 378
影响因子：
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作者：
Shingo Saito
通讯作者：
Shingo Saito
N.Iki：“通过毛细管电泳在飞摩尔水平上高效分离和测定 4-(2-吡啶偶氮基)间苯二酚的 Co(III) 和 Ni(II) 螯合物”Chem.Lett。
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