Rapid identification and quantitation for oral bacteria based on short-end capillary electrophoresis
Rapid identification and quantitation for oral bacteria based on short-end capillary electrophoresis
复制标题
基于短端毛细管电泳的口腔细菌快速鉴定与定量
DOI：
10.1016/j.talanta.2016.07.049
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发表时间：
2016-11-01
期刊：
影响因子：
6.1
通讯作者：
Dou, Xiaoming
中科院分区：
文献类型：
--
作者：
Chen, Jin;Ni, Yi;Dou, Xiaoming
高速毛细管电泳（HSCE）是一种极具发展前景的生物分子（如核酸、蛋白质）超快速、高效分析技术。本研究采用短端毛细管电泳联用一种新型空间域内标法（SDIS）对牙龈卟啉单胞菌（Porphyromonas gingivalis， P.g）、密螺旋体（Treponema denticola， T.d）和连翘单株（Tannerela forsythia， T.f） 3种口腔细菌的特异性基因进行了快速、同步分析。对该方法的可靠性、重现性和准确性进行了详细的研究。结果表明，利用该系统可以在95 s内准确、快速地鉴定和定量P.g、T.d和T.f的目的基因片段。分析物浓度与空间域信号（目标样品与内标样品之间）的比值通过SDIS方法计算出良好的线性关系。P.g、T.d、T.f基因的相关系数R-2分别为0.9855、0.9896、0.9969和0.077、0.114和0.098 ng/mu l。(C) 2016 Elsevier B.V.版权所有
High-speed capillary electrophoresis (HSCE) is a promising technology applied in ultra-rapid and high-performance analysis of biomolecules (such as nucleic acids, protein). In present study, the short-end capillary electrophoresis coupled with one novel space domain internal standard method (SDIS) was employed for the rapid and simultaneous analysis of specific genes from three oral bacteria (Porphyromonas gingivalis (P.g), Treponema denticola (T.d) and Tannerela forsythia (T.f)). The reliability, reproducibility and accuracy properties of above mentioned SDIS method were investigated in detail. The results showed the target gene fragments of P.g, T.d and T.f could be precisely, fast identified and quantitated within 95 s via present short-end CE system. The analyte concentration and the ratio of space domain signals (between target sample and internal standard sample) featured a well linear relationship calculated via SDIS method. And the correlation coefficients R-2 and detection limits for P.g, T.d, T.f genes were 0.9855, 0.9896, 0.9969 and 0.077, 0.114 and 0.098 ng/mu l, respectively. (C) 2016 Elsevier B.V. All rights reserved.