A protein secretion system linked to bacteroidete gliding motility and pathogenesis
A protein secretion system linked to bacteroidete gliding motility and pathogenesis
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DOI：
10.1073/pnas.0912010107
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发表时间：
2010-01-05
影响因子：
11.1
通讯作者：
Nakayama, Koji
中科院分区：
文献类型：
--
作者：
Sato, Keiko;Naito, Mariko;Nakayama, Koji
牙龈卟啉单胞菌分泌称为牙龈菌蛋白酶的强蛋白酶，其涉及牙周病发病机制。牙龈卟啉单胞菌中缺乏其他革兰氏阴性菌共有的蛋白质分泌系统，但包括PortT在内的几种蛋白质与牙龈菌蛋白酶分泌有关。比较基因组分析和遗传实验揭示了另外11种参与牙龈菌蛋白酶分泌的蛋白质。其中六个（PorK，PorL，PorM，PorN，PorW和Sov）在序列上与约氏黄杆菌滑动运动蛋白相似，另外两个（PorX和PorY）是推定的双组分系统调节蛋白。荧光定量RT-PCR分析显示porK、porL、porM、porN、porP、porT和sov在牙龈卟啉单胞菌porX和porY突变体中表达下调。F. johnsoniae portT直系同源物导致运动缺陷、几丁质酶分泌和滑动运动蛋白SprB粘附素移位至细胞表面，提供了拟杆菌门成员中独特的蛋白质移位系统和运动装置之间的联系。
Porphyromonas gingivalis secretes strong proteases called gingipains that are implicated in periodontal pathogenesis. Protein secretion systems common to other Gram-negative bacteria are lacking in P. gingivalis, but several proteins, including PorT, have been linked to gingipain secretion. Comparative genome analysis and genetic experiments revealed 11 additional proteins involved in gingipain secretion. Six of these (PorK, PorL, PorM, PorN, PorW, and Sov) were similar in sequence to Flavobacterium johnsoniae gliding motility proteins, and two others (PorX and PorY) were putative two-component system regulatory proteins. Real-time RT-PCR analysis revealed that porK, porL, porM, porN, porP, porT, and sov were down-regulated in P. gingivalis porX and porY mutants. Disruption of the F. johnsoniae porT ortholog resulted in defects in motility, chitinase secretion, and translocation of a gliding motility protein, SprB adhesin, to the cell surface, providing a link between a unique protein translocation system and a motility apparatus in members of the Bacteroidetes phylum.