Fast nonlinear spectral microscopy of in vivo human skin.
Fast nonlinear spectral microscopy of in vivo human skin.
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DOI：
10.1364/boe.2.000365
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发表时间：
2011-01-18
影响因子：
3.4
通讯作者：
Gerritsen HC
中科院分区：
文献类型：
--
作者：
Bader AN;Pena AM;Johan van Voskuilen C;Palero JA;Leroy F;Colonna A;Gerritsen HC
一个优化的系统，快速，高分辨率的光谱成像在体内人体皮肤的开发和评估。光谱仪由色散棱镜和电子倍增CCD摄像机组成。自发荧光和二次谐波产生（SHG）的光谱以8kHz的速率获得，并且光谱图像在几秒钟内获得。通过同时记录背景光谱，图像质量显著提高。采集224 × 224像素的体内光谱图像，背景校正并在6.5秒内以真实的RGB颜色预览。观察到体内人皮肤中更深的表皮层中的黑色素含量明显增加。
An optimized system for fast, high-resolution spectral imaging of in vivo human skin is developed and evaluated. The spectrograph is composed of a dispersive prism in combination with an electron multiplying CCD camera. Spectra of autofluorescence and second harmonic generation (SHG) are acquired at a rate of 8 kHz and spectral images within seconds. Image quality is significantly enhanced by the simultaneous recording of background spectra. In vivo spectral images of 224 × 224 pixels were acquired, background corrected and previewed in real RGB color in 6.5 seconds. A clear increase in melanin content in deeper epidermal layers in in vivo human skin was observed.