In vivo biodistribution and accumulation of 89Zr in mice.
In vivo biodistribution and accumulation of 89Zr in mice.
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DOI：
10.1016/j.nucmedbio.2010.12.011
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发表时间：
2011-07
影响因子：
3.1
通讯作者：
Smith-Jones PM
中科院分区：
文献类型：
--
作者：
Abou DS;Ku T;Smith-Jones PM
目前的调查重点是89锆在小鼠的化学和生物命运。本文介绍了~（89）Zr在不同条件下的溶剂化和螯合物的电泳。本文用~（89）Zr草酸盐、~（89）Zr氯化物、~（89）Zr磷酸盐、~（89）Zr去铁胺盐和~（89）Zr柠檬酸盐注射小鼠，通过体内分布、清除率和PET显像研究了注射后的生物学转归。还特别关注89 Zr骨蓄积的质量。在色谱纸上进行电泳，并通过γ计数读取。然后，将溶液静脉注射到小鼠中，在不同时间点成像并处死。分离骨、骨骺和骨髓，并进行γ计数。[89 Zr] Zr-氯化物和[89 Zr] Zr-草酸盐的清除率在6天后达到ID的20%，而[89 Zr] Zr-磷酸盐仅为ID的5%。[89 Zr] Zr-柠檬酸盐和[89 Zr]Zr-DFO在注射后第一天后明显排泄。注射后8 h，[89 Zr] Zr-氯化物和[89 Zr] Zr-草酸盐分别导致约15% ID/g和约20% ID/g的骨摄取。6天后损失较小。还观察到[89 Zr] Zr-柠檬酸盐骨吸收，但[89 Zr] Zr-磷酸盐在肝脏和脾脏中吸收量较高。与钙化组织相比，骨髓细胞的放射性不显著。尽管锆协调的复杂性，电泳分析提供了详细的证据，锆电荷作为盐或络合物。这项研究还表明，弱螯合的89 Zr是一种骨导向剂，对磷酸盐具有很强的亲和力。
The present investigation focuses on the chemical and biological fate of 89Zr in mice. Electrophoreses of 89Zr solvated or chelated in different conditions are here presented. The biological fate of mice injected with [89Zr]Zr-oxalate, [89Zr]Zr-chloride, [89Zr]Zr-phosphate, [89Zr]Zr-desferrioxamine and [89Zr]Zr-citrate is studied with the biodistribution, the clearances and PET images. A special focus is also given regarding the quality of 89Zr bone accumulation. Electrophoreses were carried out on chromatography paper and read by gamma counting. Then, the solutions were intravenously injected in mice, imaged at different time points and sacrificed. The bones, the epiphysis and the marrow substance were separated and evaluated with gamma counts. The clearances of [89Zr]Zr-chloride and [89Zr]Zr-oxalate reached 20% of ID after 6 days whereas [89Zr]Zr-phosphate was only 5% of ID. [89Zr]Zr-citrate and [89Zr]Zr-DFO were noticeably excreted after the first day p.i.. [89Zr]Zr-chloride and [89Zr]Zr-oxalate resulted in a respective bone uptake of ~15% ID/g and~20% ID/g at 8 h p.i. with minor losses after 6 days. [89Zr]Zr-citrate bone uptake was also observed, but [89Zr]Zr-phosphate was absorbed in high amounts in the liver and the spleen. The marrow cells were insignificantly radioactive in comparison to the calcified tissues. Despite the complexity of Zr coordination, the electrophoretic analyses provided detailed evidences of Zr charges either as salts or as complexes. This study also shows that weakly chelated, 89Zr is a bone seeker and has a strong affinity for phosphate.