A tandemly-oriented late gene cluster within the vaccinia virus genome.
A tandemly-oriented late gene cluster within the vaccinia virus genome.
复制标题
痘苗病毒基因组内串联定向的晚期基因簇。
DOI：
10.1093/nar/14.7.3003
复制
发表时间：
1986
影响因子：
14.9
通讯作者：
Hruby,DE
中科院分区：
文献类型：
--
作者：
Weinrich,SL;Hruby,DE
已经确定了来自牛痘病毒基因组中心部分的5.1内切酶对片段的核苷酸序列。在这个区域内，五个完整的和两个不完整的开放阅读框架（orfs）是紧密聚集的，串联的，并在方向上阅读。晚mRNA起始位点的五个完整的orf和一个不完整的orf确定的SI核酸酶图谱。最左边的两个完整的ORF与先前定位到该区域的65，000和32，000分子量的晚期多肽相关。当相互比较和与蛋白质数据库中的序列比较时，这五个完整的orfs在它们之间或任何先前报道的序列之间没有显着的同源性匹配。这六个推定的启动子与三个先前测序的晚期基因启动子进行比对。虽然所有九个都富含A-T，但唯一明显的共有序列是紧接在引发剂ATG之前的TAA。这种串联定向的晚期基因簇的鉴定表明病毒基因组的局部组织。
The nucleotide sequence of a 5.1 kilobase–pair fragment from the central portion of the vaccinia virus genome has been determined. Within this region, five complete and two incomplete open reading frames (orfs) are tightly–clustered, tandemly–oriented, and read in the leftward direction. Late mRNA start sites for the five complete orfs and one incomplete orf were determined by SI nuclease mapping. The two leftmost complete orfs correlated with late polypeptides of 65,000 and 32,000 molecular weight previously mapped to this region. When compared with each other and with sequences present in protein data banks, the five complete orfs showed no significant homology matches amongst themselves or any previously reported sequence. The six putative promoters were aligned with three previously sequenced late gene promoters. While all of the nine are A–T rich, the only apparent consensus sequence is TAA immediately preceeding the initiator ATG. Identification of this tandemly–oriented late gene cluster suggests local organization of the viral genome.