Use of Pollen Solid-Phase Extraction for the Determination of trans-Resveratrol in Peanut Oils
Use of Pollen Solid-Phase Extraction for the Determination of trans-Resveratrol in Peanut Oils
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利用花粉固相萃取法测定花生油中的反式白藜芦醇
DOI：
10.1021/jf505938w
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发表时间：
2015
影响因子：
6.1
通讯作者：
Feng Yu-Qi
中科院分区：
文献类型：
--
作者：
Lu Qian;Zhao Qin;Yu Qiong-Wei;Feng Yu-Qi
建立了一种简便的花粉固相萃取法测定花生油中反式白藜芦醇（TRA）的方法。首次将花粉粒作为正相固相萃取剂用于花生油中TRA的分离。花粉作为一种天然存在的物质，由于其特殊的功能结构，如羟基，饱和和不饱和脂肪链与芳香族化合物，表现出良好的吸附能力的多酚化合物。其稳定的组成以及适当的粒度（30-40 μm）也使其适用于SPE。研究了影响萃取性能的几个参数。建立了花粉柱净化-高效液相色谱-紫外检测（HPLC-UV）联用快速测定花生油中TRA的绿色净化方法。方法的线性范围为10-2500 ng·g-1，相关系数（r2）为0.9999。花生油中TRA的检出限为2.7ng·g-1，加标回收率为70.2%~ 98.4%，日内和日间相对标准偏差分别小于4.9%和5.2%。该方法已成功地应用于几种不同品牌花生油及其它植物油中TRA的分析。
In this study, a simple and convenient method for the determination oftrans-resveratrol (TRA) in peanut oils based on pollen grain solid-phase extraction (SPE) was developed. Pollen grains were used as normal-phase SPE sorbent to separate TRA from peanut oils for the first time. As a naturally occurring material, pollen grains exhibited an excellent adsorption capacity for polyphenolic compounds due to their particular functional structures such as hydroxyl groups, saturated and unsaturated aliphatic chains with aromatics. Their stable compositions as well as adequate particle size (30–40 μm) also make them suitable for SPE. Several parameters influencing extraction performance were investigated. Coupled with high-performance liquid chromatography-ultraviolet detection (HPLC-UV), a green purification method for fast determination of TRA in peanut oils using pollen grain cartridges as sorbents was established. The linearity range of the proposed method was 10–2500 ng·g–1with a satisfactory correlation coefficient (r2) of 0.9999. The limit of detection (LOD) for TRA in peanut oils was 2.7 ng·g–1, and the recoveries in spiked oil samples were from 70.2% to 98.4% with the relative standard deviations (RSDs) less than 4.9% (intraday) and 5.2% (interday). This method was successfully applied to the analysis of TRA in several peanut oils with different brands from local market as well as other kinds of vegetable oils.