Remyelination of demyelinated CNS axons by transplanted human Schwann cells: The deleterious effect of contaminating fibroblasts
Remyelination of demyelinated CNS axons by transplanted human Schwann cells: The deleterious effect of contaminating fibroblasts
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DOI：
10.3727/000000001783986774
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发表时间：
2001-01-01
影响因子：
3.3
通讯作者：
Blakemore, WF
中科院分区：
文献类型：
--
作者：
Brierley, CMH;Crang, AJ;Blakemore, WF
通过移植形成髓鞘的细胞，脱髓鞘区域可以重新髓鞘化。施万细胞是外周神经系统中自然的重新髓鞘化细胞，具有许多特性，这可能使它们对多发性硬化症的细胞植入疗法具有吸引力，在多发性硬化症中，施万细胞自发但有限的重新髓鞘化已有充分记录。施万细胞可以在体外扩增，可能提供自体移植的机会；而且它们在多发性硬化症中可能也不会受到脱髓鞘过程的影响。尽管大鼠、猫和猴的施万细胞已被移植到啮齿动物的脱髓鞘病变中，但移植的人类施万细胞的行为尚未得到评估。在这项研究中，我们研究了将人类施万细胞注射到成年大鼠脊髓急性脱髓鞘区域的后果。我们发现，含有大量成纤维细胞污染的移植物导致大量胶原蛋白沉积和广泛的轴突退变。然而，使用抗人低亲和力神经生长因子受体抗体通过阳性免疫选择纯化的施万细胞制剂（其中成纤维细胞含量低于10%）与重新髓鞘化有关。这一结果表明，人类施万细胞的成纤维细胞污染比以往研究中所认识到的问题更严重。
Areas of dermyelination can be remyelinated bp transplanting myelin-forming cells. Schwann cells are the naturally remyelinating cells of the peripheral nervous system and have a number of features that may make them attractive for cell implantation therapies in multiple sclerosis, in which spontaneous but limited Schwann cell remyelination has been well documented. Schwann cells can be expanded in vitro, potentially affording the opportunity of autologous transplantation; and they might also be spared the demyelinating process in multiple sclerosis. Although rat, cat, and monkey Schwann cells have been transplanted into rodent demyelinating lesions, the behavior of transplanted human Schwann cells has not been evaluated. In this study we examined the consequences of injecting human Schwann cells into areas of acute demyelination in the spinal cords of adult rats. We found that transplants containing significant fibroblast contamination resulted in deposition of targe amounts of collagen and extensive axonal degeneration. However, Schwann cell preparations that had been purified by positive immunoselection using antibodies to human low-affinity nerve growth factor receptor containing less than 10% fibroblasts were associated with remyelination. This result indicates that fibroblast contamination of human Schwann cells represents a greater problem than would have been appreciated from previous studies.