Effects of photobleaching on selected advanced glycation end products in the human lens.
Effects of photobleaching on selected advanced glycation end products in the human lens.
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DOI：
10.1186/s13104-015-0977-3
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发表时间：
2015-01-16
影响因子：
1.8
通讯作者：
Kessel L
中科院分区：
文献类型：
--
作者：
Holm T;Raghavan CT;Nahomi R;Nagaraj RH;Kessel L
白内障是致盲的主要原因，尤其是在发展中国家。为了方便治疗，我们提出白内障可以通过光漂白负责白内障形成的化学修饰蛋白进行非侵入性治疗。本研究旨在研究所提出的处理方法的光学和生化效应。使用445 nm连续波激光对人体供体透镜进行光漂白。通过光透射和散射对漂白前后的透镜光学质量进行评价。测定了晚期糖基化终产物（AGEs）戊苷、银嘧啶、羧甲基赖氨酸、氢咪唑酮的浓度。光漂白后，透射率增加，age相关荧光明显下降，但测定的AGEs浓度没有变化。我们发现光漂白处理对透镜光学参数有显著影响，但我们不能将光学结果与我们测量的AGEs浓度的变化联系起来。这一发现表明，其他AGEs对激光治疗后观察到的人类晶状体的光漂白负责。与光漂白相关的光化学反应的生化性质仍有待阐明。
Cataract is the leading cause of blindness, especially in the developing world. To ease access to treatment, we have proposed that cataract could be treated non-invasively by photobleaching of the chemically modified proteins responsible for cataract formation. The present study was aimed at examining the optical and biochemical effects of the proposed treatment. Human donor lenses were photobleaced using a 445 nm cw laser. Lens optical quality was assessed before and after photobleaching by light transmission and scattering. The concentration of the advanced glycation end products (AGEs) pentosidine, argpyrimidine, carboxymethyllysine, hydroimidazolone was measured. Transmission increased and AGE-related fluorescence decreased significantly after photobleaching but no changes were observed in the concentration of the measured AGEs. We found a significant effect of the photobleaching treatment on lens optical parameters but we could not associate the optical findings to a change in the concentration of the AGEs we measured. This finding suggests that other AGEs were responsible for the observed photobleaching of the human lens after laser treatment. The biochemical nature of the photochemical reactions associated with photobleaching remains to be elucidated.