Measuring antibody responses to a live attenuated influenza vaccine in children
Measuring antibody responses to a live attenuated influenza vaccine in children
复制标题
DOI：
10.1097/01.inf.0000137566.87691.3b
复制
发表时间：
2004-09-01
影响因子：
3.6
通讯作者：
Mendelman, PM
中科院分区：
文献类型：
--
作者：
Lee, MS;Mahmood, K;Mendelman, PM
背景：血凝抑制试验(HAI)是评价灭活流感疫苗的标准方法，但目前尚无评价减毒活疫苗(LAIV)的标准方法。含有A/Beijing/262/95(H1N1)的法律在血清学研究中诱导对抗原性变异株A/新喀里多尼亚/20/99(H1N1)的低血清HAI抗体反应，但在社区研究中提供对A/新喀里多尼亚类病毒的保护。通过检测儿童对甲型H1N1流感的交叉反应抗体反应，比较中和试验和HAI试验。方法：采集50名1-8岁儿童在接种疫苗前和每次接种LAIV后4-6周的血清。用HAI和中和试验检测对3种疫苗病毒的抗体滴度，而对H1N1疫苗病毒(A/Beijing/262/95)和两种H1N1抗原变异株(A/深圳/227/95和A/新喀里多尼亚/20/99)的抗体滴度通过HAI和中和试验测定。结果：最初血清阴性的参与者对3种疫苗病毒产生HAI血清转换反应的可能性比基线血清阳性的参与者更高(H1N1的77%对14%，H3N2的100%对20%，13，P
Background: Hemagglutination inhibition (HAI) assay is the standard method for evaluating inactivated influenza vaccines, but no standard assay has been established for evaluating live attenuated influenza vaccines (LAIV). LAW containing A/Beijing/262/95(H1N1) induced low serum HAI antibody responses to the antigenic variant, A/New Caledonia/20/99(H1N1) in a serologic study but provided protection against the A/New Caledonia-like viruses in a community study. Neutralization and HAI assays were compared by measuring H1N1 cross-reactive antibody responses to the LAW in children.Methods: Sera were collected from 50 children 1-8 years of age before vaccination and 4-6 weeks after each dose of the LAIV. Antibody titers to the 3 vaccine viruses were measured by the HAI assay, whereas antibody titers against the H1N1 vaccine virus (A/Beijing/262/95) and 2 H1N1 antigenic variants (A/Shenzhen/227/95 and A/New Caledonia/20/99) were measured by the HAI and neutralization assays.Results: Initially seronegative participants were more likely to develop HAI seroconversion responses to the 3 vaccine viruses than the baseline seropositive participants (77% versus 14% for H1N1, 100% versus 20% for H3N2, 100% versus 19% for 13, P