LONG LATENCY PRECEDES OVERT SEROCONVERSION IN SEXUALLY TRANSMITTED HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION
LONG LATENCY PRECEDES OVERT SEROCONVERSION IN SEXUALLY TRANSMITTED HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION
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DOI：
10.1016/s0140-6736(87)92985-0
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发表时间：
1987-09
期刊：
影响因子：
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通讯作者：
A. Ranki;Minerva Krohn;J. Allain;G. Franchini;S. Valle;J. Antonen;M. Leuther;K. Krohn
中科院分区：
文献类型：
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作者：
A. Ranki;Minerva Krohn;J. Allain;G. Franchini;S. Valle;J. Antonen;M. Leuther;K. Krohn
研究人员在显性血清转化前收集的储存血清样本中寻找潜伏 HIV 感染的迹象，并通过酶联免疫吸附测定 (ELISA) 证实，这些样本来自 9 名人类免疫缺陷病毒 (HIV) 感染受试者、HIV 血清阳性男性的 25 名血清阴性性伴侣和其他 23 名血清阴性、同性恋活跃男性的血清。在血清转化前 6-14 个月，在所有 9 名血清转化受试者中观察到游离的 HIV 抗原和/或针对重组结构（核心、env）或非结构（3'orf、sor、tat）蛋白的低滴度抗体。通过蛋白质印迹检测到的针对核心蛋白的抗体通常是潜在 HIV 感染的第一个迹象。 25 名 ELISA 阴性暴露伴侣中，有 5 名已表现出 HIV 抗原血症和针对核心蛋白的抗体，持续 16-34 个月。通过原位杂交，在一些潜伏感染伴侣的外周血非淋巴细胞单核细胞中检测到了 HIV 特异性 RNA。所有潜在 HIV 感染者的 T4 淋巴细胞数量均正常，但其中一半的人失去了对记忆抗原（结核菌素的纯化蛋白衍生物）的体外增殖性 T 细胞反应。早期 HIV 感染的特点是对 HIV 核心和调节蛋白的低水平且有限的抗体反应，似乎主要影响抗原呈递细胞。
Signs of latent HIV infection were sought in stored serum samples collected before overt seroconversion, confirmed by enzyme-linked immunosorbent assay (ELISA), from 9 subjects with human-immunodeficiency-virus (HIV) infection, in serum from 25 seronegative sexual partners of HIV-seropositive men and from 23 other seronegative, homosexually active men. Free HIV antigen and/or low-titre antibodies to recombinant structural (core, env) or non-structural (3'orf,sor, tat) proteins were seen 6-14 months before seroconversion in all 9 subjects who seroconverted. Antibodies against core proteins detected by western blot were usually the first sign of latent HIV infection. 5 of the 25 ELISA-negative exposed partners have shown HIV antigenaemia and antibodies against core proteins for 16-34 months. By in-situ hybridisation, HIV-specific RNA was detected in peripheral-blood non-lymphoid mononuclear cells in some of the latently infected partners. All subjects with latent HIV infection had normal numbers of T4 lymphocytes but half of them lost their in-vitro proliferative T-cell response to a recall antigen (purified protein derivative of tuberculin). Early HIV infection, characterised by a low-level and restricted antibody response towards HIV core and regulatory proteins, seems mainly to affect antigen-presenting cells.