Haloarcula mannanilytica sp. nov., a galactomannan-degrading haloarchaeon isolated from commercial salt
Haloarcula mannanilytica sp. nov., a galactomannan-degrading haloarchaeon isolated from commercial salt
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DOI：
10.1099/ijsem.0.004535
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发表时间：
2020-01-01
影响因子：
2.8
通讯作者：
Minegishi, Hiroaki
中科院分区：
文献类型：
--
作者：
Enomoto, Shigeaki;Shimane, Yasuhiro;Minegishi, Hiroaki
从市售食盐样品中分离到一株能降解甘露聚糖的嗜盐古菌MD 130 -1（T）。细胞呈运动性、杆状和染色革兰氏阴性。菌落呈粉红色。菌株MD 130 -1（T）能够在1.5-4.6 M NaCl（最适，3.6 M）、pH 6.0-8.0（最适，pH 7.0）和25-50 ℃（最适，40 ℃）下生长。DNA G+C含量为62.1mol%（基因组）。其16 S rRNA基因序列与日本盐生菌（Haloarcula japonica）JCM 7785（T）和西班牙盐生菌（Haloarcula hispanica）JCM 8911（T）的同源性最高（99.4%）。菌株MD 130 -1（T）与盐节藻属物种的基因组相关性在ANIb中为84.33- 85.96%，在GGDC公式2中为30.4-32.9%。菌株MD 130 -1（T）的极性脂质为磷脂酰甘油、磷脂酰甘油磷酸甲酯和三糖基二醚-2。根据表型和系统发育分析的结果，该菌株代表了一个新的种，并建议将其命名为Haloarcula mannanilytica sp. nov.。模式菌株是从日本石川县生产的商品盐中分离的MD 130 -1（T）（=JCM 33835（T）=KCTC 4287（T））。
A mannan-degrading halophilic archaeal strain, MD130-1(T), was isolated from a commercial salt sample. Cells were motile, rod-shaped, and stained Gram-negative. Colonies were pink pigmented. Strain MD130-1(T) was able to grow at 1.5-4.6 M NaCl (optimum, 3.6 M) at pH 6.0-8.0 (optimum, pH 7.0) and at 25-50 degrees C (optimum, 40 degrees C). The DNA G+C content was 62.1 mol% (genome). The orthologous 16S rRNA gene sequence showed the highest similarity (99.4 %) to those of Haloarcula japonica JCM 7785(T) and Haloarcula hispanica JCM 8911(T). The values of genome relatedness between strain MD130-1(T) and Haloarcula species were 84.33-85.96 % in ANIb and 30.4-32.9% using GGDC formula 2. The polar lipids of strain MD130-1(T) were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and triglycosyl diether-2. Based on the results of phenotypic and phylogenetic analyses, the strain represents a new species of the genus Haloarcula, for which the name Haloarcula mannanilytica sp. nov. is proposed. The type strain is MD130-1(T) (=JCM 33835(T)=KCTC 4287(T)) isolated from commercial salt made in Ishikawa prefecture, Japan.