Influence of morphology on the near-infrared spectra of mycelial biomass and its implications in bioprocess monitoring.
Influence of morphology on the near-infrared spectra of mycelial biomass and its implications in bioprocess monitoring.
复制标题
形态对菌丝生物量近红外光谱的影响及其在生物过程监测中的意义。
DOI：
--
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发表时间：
2003
影响因子：
3.8
通讯作者：
B. McNeil
中科院分区：
文献类型：
--
作者：
S. Vaidyanathan;S. White;L. Harvey;B. McNeil
Bioprocesses that employ mycelial microorganisms are commercially important. The application of optical techniques for the measurement of biomass in such processes is limited by the morphological heterogeneity exhibited by the mycelial microorganism employed. We investigated the influence of morphology on the near-infrared (NIR) spectra of the biomass of Streptomyces fradiae, a filamentous microorganism, by studying the spectra of mycelial suspensions that were manipulated to generate a range of morphological forms. Computerized image analysis was used to characterize the morphological forms. Principal component analysis was used to assess the spectral variations and study correlations to the manipulated mycelial morphology. Although morphology was found to influence the near infrared transmittance spectra of biomass, the influence was less pronounced than in the visible region, the spectral information at longer wavelengths (1600-2350 nm) showing greater stability to morphological variations. Long-wave NIR spectral information is therefore likely to be more useful in estimating biomass in mycelial bioprocesses. Furthermore, the NIR reflectance spectra of dried biomass were found to show correlations to the morphological variations introduced, suggesting that NIR spectra may be useful in obtaining morphology related information.
