Regional Conjunctival Differences in Glycocalyx Mucin Expression in Dry Eye and Normal Subjects.
Regional Conjunctival Differences in Glycocalyx Mucin Expression in Dry Eye and Normal Subjects.
复制标题
干眼症和正常受试者糖萼粘蛋白表达的区域结膜差异。
DOI：
10.1167/iovs.65.2.20
复制
发表时间：
2024
影响因子：
4.4
通讯作者：
Tichenor,AnnaAblamowicz
中科院分区：
文献类型：
--
作者：
Choi,Moonjung;Tichenor,AnnaAblamowicz
目的：比较正常和干眼 (DE) 受试者中膜相关粘蛋白 (MAM) MUC1、MUC4 和 MUC16 的区域结膜表达。方法：招募有或没有 DE 体征和症状的成年人。进行印模细胞学检查，从双眼的四个球部和上眼睑睑结膜区域收集 MAM。蛋白质提取后，按区域汇集单个受试者双眼的样本，并使用毛细管电泳纳米免疫分析系统分析表达。标准化至总蛋白量后计算每个抗原结合信号的化学发光强度。使用 GraphPad Prime 9 进行统计分析。结果：分析了 13 至 16 个 DE 和 7 至 11 个正常受试者的样本。在正常样本中，鼻球结膜的MUC1表达显着高于上（P = 0.004）和下（P = 0.005）。在DE样本中，MUC1表达最高。在正常样本中未观察到 MUC4 和 MUC16 表达的显着差异。与正常样本中的这些区域相比，DE 中的 MUC4 和 MUC16 表达量上调（P < 0.0001）和下调（P < 0.0001）。 结论：虽然 MAM 形成称为糖萼的亲水屏障，但每种粘蛋白可能具有目前尚未探索的独特功能。所有 MAM 均在上睑结膜中表达。健康受试者鼻腔 MUC1 表达增加表明功能需要增加保护。当将 DE 与正常眼睛进行比较时，MUC1 的上调以及 MUC4 和 MUC16 的上调和下调可能表明需要减少眨眼期间的眼睑摩擦，尤其是在 DE 中。
Purpose: To compare regional conjunctival expression of membrane-associated mucins (MAMs) MUC1, MUC4, and MUC16 in normal and dry eye (DE) subjects.Methods: Adults with and without signs and symptoms of DE were recruited. Impression cytology was performed to collect MAMs from four bulbar and upper eyelid palpebral conjunctival regions of both eyes. After protein extraction, samples from both eyes of a single subject were pooled by region, and expression was analyzed using a capillary electrophoresis nano-immunoassay system. The chemiluminescence intensity of each antigen binding signal was calculated after normalization to the total protein amount. Statistical analyses were conducted using GraphPad Prime 9.Results: Samples from thirteen to sixteen DE and seven to eleven normal subjects were analyzed. In normal samples, MUC1 expression from the nasal bulbar conjunctiva was significantly greater than superior (P= 0.004) and inferior (P= 0.005). In DE samples, MUC1 expression was highest superiorly. Significant differences in MUC4 and MUC16 expression were not seen in normal samples. MUC4 and MUC16 expression was upregulated superiorly (P< 0.0001) and inferiorly (P< 0.0001) in DE compared with those regions in normal samples.Conclusions: Although MAMs form a hydrophilic barrier called the glycocalyx, each mucin may have unique functions that are currently unexplored. All MAMs were expressed in the upper palpebral conjunctiva. Increased MUC1 expression nasally in healthy subjects suggests a functional need for increased protection. When comparing DE with normal eyes, upregulation of MUC1 superiorly, and in both MUC4 and MUC16 both superiorly and inferiorly, may indicate a need to decrease eyelid friction during blinking, especially in DE.