Primary Metabolism, Phenylpropanoids and Antioxidant Pathways Are Regulated in Potato as a Response to Potato virus Y Infection.
Primary Metabolism, Phenylpropanoids and Antioxidant Pathways Are Regulated in Potato as a Response to Potato virus Y Infection.
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DOI：
10.1371/journal.pone.0146135
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Gruden K
中科院分区：
文献类型：
--
作者：
Kogovšek P;Pompe-Novak M;Petek M;Fragner L;Weckwerth W;Gruden K
马铃薯生产是最重要的农业部门之一，它受到各种有害因素的挑战，包括病毒感染。为了控制马铃薯生产中的损失，了解病毒-植物相互作用至关重要。在这里，我们调查了马铃薯Y病毒（PVY），最重要的经济马铃薯病毒病原体感染的结果在马铃薯植物的分子过程。我们进行了一项综合研究，在马铃薯叶片接种温和PVYN和侵略性PVYNTN菌株，通过疾病发展的不同时间的代谢组和基因表达的变化。在感染开始时（接种后1天），病毒感染的植物表现出初步减少的浓度的代谢产物连接到糖和氨基酸代谢，TCA循环，GABA分流，活性氧清除剂，和苯丙素，相对于对照植物。这些代谢物的显着增加，检测到在感染的叶子开始强烈的病毒增殖。通过定量PCR分析，在基因表达水平上也观察到这些代谢途径的改变。此外，还分析了代谢组对PVY感染的全身反应。系统叶表现出不太明显的反应，较少的代谢物改变，而苯丙酸相关的代谢物强烈积累。接种PVYN的叶片中活性氧清除剂的积累比接种PVYNTN的叶片中活性氧清除剂的积累开始更快。这似乎与用较温和的PVYN菌株感染的马铃薯叶观察到的较低损伤有关，并且至少部分地解释了观察到的表型之间的差异。
Potato production is one of the most important agricultural sectors, and it is challenged by various detrimental factors, including virus infections. To control losses in potato production, knowledge about the virus—plant interactions is crucial. Here, we investigated the molecular processes in potato plants as a result of Potato virus Y (PVY) infection, the most economically important potato viral pathogen. We performed an integrative study that links changes in the metabolome and gene expression in potato leaves inoculated with the mild PVYN and aggressive PVYNTN isolates, for different times through disease development. At the beginning of infection (1 day post-inoculation), virus-infected plants showed an initial decrease in the concentrations of metabolites connected to sugar and amino-acid metabolism, the TCA cycle, the GABA shunt, ROS scavangers, and phenylpropanoids, relative to the control plants. A pronounced increase in those metabolites was detected at the start of the strong viral multiplication in infected leaves. The alterations in these metabolic pathways were also seen at the gene expression level, as analysed by quantitative PCR. In addition, the systemic response in the metabolome to PVY infection was analysed. Systemic leaves showed a less-pronounced response with fewer metabolites altered, while phenylpropanoid-associated metabolites were strongly accumulated. There was a more rapid onset of accumulation of ROS scavengers in leaves inoculated with PVYN than those inoculated with PVYNTN. This appears to be related to the lower damage observed for leaves of potato infected with the milder PVYN strain, and at least partially explains the differences between the phenotypes observed.