An implant labeling technique employing sable hair probes as carriers for 3H-thymidine: applications to the study of facial morphogenesis.
An implant labeling technique employing sable hair probes as carriers for 3H-thymidine: applications to the study of facial morphogenesis.
复制标题
使用貂毛探针作为 3H-胸苷载体的植入物标记技术：在面部形态发生研究中的应用。
DOI：
10.1002/ar.1092100313
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Minkoff,R
中科院分区：
文献类型：
--
作者：
Patterson,SB;Johnston,MC;Minkoff,R
一种植入标记技术是描述利用貂毛探针作为载体的氚化胸腺嘧啶标记。所开发的方案产生了特定胚胎细胞群体的局部标记。该方法应用于鸡胚面部发育的分析。对该技术的评估表明，探针制备过程在生产标记探针方面始终是成功的。使用标记探针，该程序在鸡胚胎组织中产生可接受水平的标记是可靠的，并且可以使用植入标记技术标记局部细胞群。在标记中心周围，经常观察到标记强度的梯度。这种模式可能反映了随着距离探针的增加，标记胸苷的可用性下降。如果遵循无菌程序，该技术可以获得极好的存活率。此外，对较老胚胎的仔细分析未能揭示由植入物标记程序引起的任何畸形。在本研究中显示的局部标记模式表明，植入物标记技术将为跟踪面部突起形成过程中的细胞迁移提供有用的工具。
An implant labeling technique is described that utilizes sable hair probes as carriers for a tritiated thymidine marker. The protocol that was developed produced localized labeling of specific embryonic cell populations. This procedure was applied to the analysis of facial process development in chick embryos.Evaluation of the technique demonstrated that the probe preparation procedure was consistently successful in producing labeled probes. Using labeled probes, the procedure was reliable in producing acceptable levels of labeling in chick embryonic tissues and labeling of localized cell populations was possible using the implant labeling technique. Surrounding the center of labeling, a gradient in intensity of labeling was often observed. This pattern presumably reflects declining availability of labeled thymidine as distance from the probe increased. The technique allowed excellent survival rates to be achieved provided that aseptic procedures were followed. Additionally, careful analysis of older embryos failed to reveal any malformations induced by the implant labeling procedure.The localized labeling patterns that were demonstrated during this investigation suggest that the implant labeling technique would provide a useful tool for following cell migration during facial process formation.