INVITRO METABOLISM OF 25-HYDROXYCHOLECALCIFEROL BY ISOLATED CELLS FROM HUMAN DECIDUA
INVITRO METABOLISM OF 25-HYDROXYCHOLECALCIFEROL BY ISOLATED CELLS FROM HUMAN DECIDUA
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DOI：
10.1210/jcem-60-5-880
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发表时间：
1985-01-01
影响因子：
5.8
通讯作者：
MAMER, OA
中科院分区：
文献类型：
--
作者：
DELVIN, EE;ARABIAN, A;MAMER, OA
母体血清中1 α水平的增加，25-妊娠期间服用二羟钙化醇与肠钙吸收增强有关。在不同物种中提出了其合成的几个位点，人类蜕膜就是其中之一。从足月胎盘分离的胶原酶分散的蜕膜细胞在Percoll梯度上分级。将分离的细胞置于6 nM [3H]胆钙化醇存在下培养。两个形态相似的细胞群水解底物，产生一种化合物，其质谱与真正的1 α相同，25-二羟基胆钙化醇在4个色谱系统中，并结合到特定的佝偻病鸡受体。这些制剂提供了一个潜在的系统，通过该系统可以在体外研究人胎盘合成激素形式维生素D的动力学和调节。
An increase in maternal serum levels of 1.alpha.,25-dihydroxycalciferol during pregnancy was linked to enhancement of intestinal Ca absorption. Several sites of its synthesis were proposed in different species, human decidua being one of them. Collagenase-dispersed decidual cells isolated from term placenta were fractionated on a Percoll gradient. The isolated cells were set in culture in the presence of 6 nM [3H]cholecalciferol. Two cell populations of similar morphology hydrolyzed the substrate, yielding a compound that had a mass spectrum identical to and that comigrated with authentic 1.alpha.,25-dihydroxycholecalciferol in 4 chromatographic systems and bound to a specific rachitic chick receptor. These preparations provide a potential system by which the kinetics and regulation of the synthesis of the hormonal form of vitamin D by human placenta can be studied in vitro.