QUALITY-CONTROL IN TUBERCULOSIS BACTERIOLOGY .1. LABORATORY STUDIES ON ISOLATED POSITIVE CULTURES AND THE EFFICIENCY OF DIRECT SMEAR EXAMINATION
QUALITY-CONTROL IN TUBERCULOSIS BACTERIOLOGY .1. LABORATORY STUDIES ON ISOLATED POSITIVE CULTURES AND THE EFFICIENCY OF DIRECT SMEAR EXAMINATION
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DOI：
10.1016/0041-3879(80)90001-x
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
KEYES, AB
中科院分区：
文献类型：
--
作者：
ABER, VR;ALLEN, BW;KEYES, AB
对不太可能含有结核分枝杆菌的痰液标本[人]或人工制备为甲基纤维素/鸡蛋溶液的标本进行高压灭菌，并与含有活M的痰液标本一起分批分发。结核病具有不寻常的药物敏感性模式。这些标本在1个东非实验室制备，分成等分试样，并在3个东非实验室通过直接涂片和培养进行检查。还在伦敦[英格兰]制备并培养了独立批次。少量的M.从一定比例的高压灭菌标本中获得的结核病总具有特征性标记物敏感性模式，范围从伦敦825份标本的0%到坎帕拉[乌干达] 2165份标本的1.3%。杆菌从阳性标本转移到阴性标本可以解释临床试验中出现的一些分离阳性培养物。转移的发生似乎更多地与技术人员的素质有关，而不是与技术方法的细节有关。非洲实验室在涂片和培养检查方面的总体效率可以分级。
Sputum specimens [human] unlikely to contain Mycobacterium tuberculosis or specimens artificially created as methylcellulose/egg solution were autoclaved and distributed in batches together with sputum specimens containing viable M. tuberculosis marked with an unusual drug-sensitivity pattern. These specimens were prepared in 1 East African laboratory, divided into aliquots and examined by direct smear and culture in 3 East African laboratories. Independent batches were also prepared and cultured in London [England]. Scanty colonies of M. tuberculosis always with the characteristic marker sensitivity pattern, were obtained from a proportion of the autoclaved specimens, ranging from 0% of 825 specimens in London to 1.3% of 2165 specimens in Kampala [Uganda]. Transfer of bacilli from positive to negative specimens could account for some of the isolated positive cultures that occur in clinical trials. The occurrence of transfer seemed to be related more to the quality of the technician than to the detail of the technical methods. The African laboratories could be graded in their overall efficiency in smear and culture examination.