One-pot fluorescence detection of multiple analytes in homogenous solution based on noncovalent assembly of single-walled carbon nanotubes and aptamers.
One-pot fluorescence detection of multiple analytes in homogenous solution based on noncovalent assembly of single-walled carbon nanotubes and aptamers.
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DOI：
10.1016/j.bios.2011.01.035
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发表时间：
2011-04
影响因子：
12.6
通讯作者：
Yuanfu Zhang;Baoxin Li;C. Yan;Lihui Fu
中科院分区：
文献类型：
--
作者：
Yuanfu Zhang;Baoxin Li;C. Yan;Lihui Fu
We have developed a new multicolor fluorescent sensing system to detect multiple analytes in one pot. This design is based on the noncovalent assembly of dye-labeled aptamer with single-walled carbon nanotubes (SWNTs) by π-stacking between the nucleotide bases and the SWNTs sidewalls. In the presence of the targets, the aptamer–target binding separates the assembly of dye-labeled aptamers and SWNTs, resulting in the restoration of fluorescence signal of the dye labeled with aptamers. As a proof of concept, we demonstrate that a two-color fluorescent system can simultaneously and selectively detect two targets (thrombin and adenosine triphosphate) in a single solution. Since the method is mix-and-detect manner, the present strategy is simple and cost-effective.
