Comparative sequence analysis of major envelope protein gene (B2L) of Indian orf viruses isolated from sheep and goats
Comparative sequence analysis of major envelope protein gene (B2L) of Indian orf viruses isolated from sheep and goats
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DOI：
10.1016/j.vetmic.2006.04.028
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发表时间：
2006-09-10
影响因子：
3.3
通讯作者：
Singh, R. K.
中科院分区：
文献类型：
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作者：
Hosamani, Madhusudan;Bhanuprakash, V.;Singh, R. K.
口蹄疫病毒（ORFV）是副痘病毒的类型种，是绵羊和山羊传染性湿疹的罪魁祸首。在本报告中，对来自绵羊和山羊的4个印度口蹄疫病毒分离株的主包膜基因（B2L）进行了序列分析。这些最近的分离株属于2004-2005年期间在Kumaon丘陵和邻近平原发生的不同疫情。采用诊断性PCR对结痂样本进行初步筛选。采用PCR方法扩增出4株ORFV分离株免疫原性主包膜蛋白全长B2L基因，克隆扩增子（1206 bp）并测序。比较序列分析显示，该开放阅读框包含1137个核苷酸（nt），编码378个氨基酸（aa）的多肽。印度分离株之间高度相关，在nt和as水平上序列同源性超过97%。此外，它们与来自世界各地的其他ORFV分离株的序列具有97-98%的序列一致性；假牛痘病毒和牛丘疹性口炎病毒的序列同源性分别为94 ~ 95和82.7 ~ 83.8%。系统发育分析还表明，这些来自绵羊和山羊的副痘病毒与世界上报道的其他口蹄疫病毒密切相关。(c) 2006 Elsevier b.v.版权所有
Orf virus (ORFV), the type species of Parapoxvirus, is responsible for contagious ecthyma in sheep and goats. In the present report, sequence analysis of major envelope gene (B2L) of four Indian orf virus isolates originating two each from sheep and goats was carried out. These recent isolates belonged to different outbreaks that occurred in Kumaon hills and adjoining plains during 2004-2005. Preliminary screening of the scab samples was carried out by diagnostic PCR. Full-length B2L gene encoding for immunogenic major envelope protein from all the four ORFV isolates was amplified by PCR and the amplicons (1206 bp) were cloned and sequenced. Comparative sequence analysis revealed an open reading frame of 1137 nucleotides (nt) encoding a polypeptide of 378 amino acids (aa). Indian isolates were highly related amongst themselves with sequence identity of over 97% at the nt and as level. Further, they showed 97-98% sequence identity with sequences of other ORFV isolates from around the world; while 94-95 and 82.7-83.8% sequence identity was observed, respectively, with pseudocowpox and bovine papular stomatitis viruses-the other members of the genus. Phylogenetic analysis also showed that these Parapoxviruses from sheep and goats are closely related to other orf viruses reported worldwide. (c) 2006 Elsevier B.V All rights reserved.