Genetic Engineering of Adipose-Derived Stem Cells Using Biodegradable and Lipid-Like Highly Branched Poly(β-amino ester)s
Genetic Engineering of Adipose-Derived Stem Cells Using Biodegradable and Lipid-Like Highly Branched Poly(β-amino ester)s
复制标题
使用可生物降解和类脂质高度支化聚（β-氨基酯）进行脂肪干细胞的基因工程
DOI：
10.1021/acsmacrolett.2c00095
复制
发表时间：
2022
影响因子：
5.8
通讯作者：
Dezhong Zhou
中科院分区：
文献类型：
--
作者：
Qiuxia Li;Litao Sun;Xiaobei Huang;Shuai Liu;Haiyang Yong;Chenfei Wang;Jianzhong Li;Dezhong Zhou
为了通过基因转染来增强脂肪干细胞的生物学功能，开发了一种可生物降解的类脂质超支化聚β-氨基酯（HPAESA）。生物降解性降低了HPAESA的细胞毒性，并使DNA释放受控。脂质模拟增强HPAESA/DNA复合物的细胞摄取和内体逃逸。HPAESA能够以比商业基因转染试剂高几个数量级的效率转染大鼠脂肪源性干细胞（rADS）和人ADSC（hADSC），细胞活力超过90%。最重要的是，HPAESA可以有效地将编码神经生长因子（NGF）的质粒转移到rADSC中，并诱导高水平的NGF分泌，从而显著促进PC 12细胞的神经突起生长。
Biodegradable and lipid-like highly branched poly(β-amino ester)s, HPAESA, were developed to enhance the biological functions of adipose-derived stem cells by gene transfection. Biodegradability reduces the cytotoxicity of HPAESA and enables controlled DNA release. Lipid mimicry enhances cellular uptake and endosomal escape of HPAESA/DNA polyplexes. HPAESA are able to transfect rat adipose-derived stem cells (rADSs) and human ADSCs (hADSCs) with orders of magnitude higher efficiency than commercial gene transfection reagents, with cell viability exceeding 90%. Most importantly, HPAESA can effectively transfer the nerve growth factor (NGF)-encoding plasmid to rADSCs and induce high NGF secretion, which significantly promotes neurite outgrowth of PC12 cells.