Comparative Analysis of the First Complete Enterococcus faecium Genome
Comparative Analysis of the First Complete Enterococcus faecium Genome
复制标题
DOI：
10.1128/jb.00259-12
复制
发表时间：
2012-05-01
影响因子：
3.2
通讯作者：
Stinear, Timothy P.
中科院分区：
文献类型：
--
作者：
Lam, Margaret M. C.;Seemann, Torsten;Stinear, Timothy P.
万古霉素耐药肠球菌（VRE）是地球仪中医院感染的主要原因之一。特别是，由耐万古霉素屎肠球菌引起的感染变得越来越常见。E.屎肠球菌分离物由于缺乏完全组装的E.粪菌基因组序列。在这里，我们解决这个问题，并报告完整的3.0 Mb基因组序列的多位点序列类型17万古霉素耐药屎肠球菌菌株Aus0004，从血液中的病人在墨尔本，澳大利亚，在1998年。基因组由一条2.9Mb的环状染色体和三个环状质粒组成。该染色体携带推定的E.肠球菌表面蛋白、溶血素和胶原结合粘附素等肠球菌毒力因子。Aus0004具有一个非常大的辅助基因组（38%），包括三个原噬菌体和两个在其他22个E.粪菌基因组其中一个原噬菌体作为反向50 kb重复序列存在，似乎促进了683 kb染色体在复制末端的反向，导致了惊人的复制素不平衡。其他显著特征包括76个插入序列元件和含有vanB万古霉素抗性元件的Tn1549的单个染色体拷贝。一个完整的E.屎肠菌基因组将是一个有用的资源，以帮助我们了解这种新兴的医院病原体。
Vancomycin-resistant enterococci (VRE) are one of the leading causes of nosocomial infections in health care facilities around the globe. In particular, infections caused by vancomycin-resistant Enterococcus faecium are becoming increasingly common. Comparative and functional genomic studies of E. faecium isolates have so far been limited owing to the lack of a fully assembled E. faecium genome sequence. Here we address this issue and report the complete 3.0-Mb genome sequence of the multilocus sequence type 17 vancomycin-resistant Enterococcus faecium strain Aus0004, isolated from the bloodstream of a patient in Melbourne, Australia, in 1998. The genome comprises a 2.9-Mb circular chromosome and three circular plasmids. The chromosome harbors putative E. faecium virulence factors such as enterococcal surface protein, hemolysin, and collagen-binding adhesin. Aus0004 has a very large accessory genome (38%) that includes three prophage and two genomic islands absent among 22 other E. faecium genomes. One of the prophage was present as inverted 50-kb repeats that appear to have facilitated a 683-kb chromosomal inversion across the replication terminus, resulting in a striking replichore imbalance. Other distinctive features include 76 insertion sequence elements and a single chromosomal copy of Tn1549 containing the vanB vancomycin resistance element. A complete E. faecium genome will be a useful resource to assist our understanding of this emerging nosocomial pathogen.