Increased osteoblast and decreased Staphylococcus epidermidis functions on nanophase ZnO and TiO2
Increased osteoblast and decreased Staphylococcus epidermidis functions on nanophase ZnO and TiO2
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DOI：
10.1002/jbm.a.30789
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发表时间：
2006-09-01
影响因子：
4.9
通讯作者：
Webster, Thomas J.
中科院分区：
文献类型：
--
作者：
Colon, Gabriel;Ward, Brian C.;Webster, Thomas J.
许多工程师和外科医生将植入物的失败归因于骨结合不良(或矫形植入物与并列的骨骼粘合)和/或细菌感染。通过使用新的纳米拓扑学，研究人员已经证明了纳米结构陶瓷、碳纤维、聚合物、金属和复合材料增强了成骨细胞的黏附和钙/磷矿物的沉积。然而，细菌在具有纳米结构表面的材料上的功能在很大程度上仍未被研究。尽管在正常的整形外科植入物的手术中，来自患者自己皮肤和/或粘膜的细菌会进入伤口部位，但这是事实。这些细菌(即表皮葡萄球菌)不可逆转地附着在种植体表面，而各种生理压力会导致细菌生长速度的变化，从而导致生物膜的形成。由于它们在决定整形外科植入物的成功中起着不可或缺的作用，这项体外研究的目的是检测(I)表皮葡萄球菌和(Ii)成骨细胞(或骨形成细胞)在氧化锌和二氧化钛(二氧化钛)上的功能，与微结构表面特征相比，这两种材料具有纳米结构。氧化锌是一种著名的抗菌剂，一旦植入钛表面，很容易形成二氧化钛。这项研究的结果提供了第一个证据，即与微结构表面特征相比，纳米结构减少了表皮葡萄球菌在氧化锌和二氧化钛上的粘附性。此外，与微相制剂相比，本研究的结果显示，成骨细胞的粘附力、碱性磷酸酶活性以及钙矿物在纳米级氧化锌和二氧化钛上的沉积都有所增加。通过这种方式，这项研究表明，纳米级的氧化锌和二氧化钛可以减少表皮葡萄球菌的粘连，增加促进骨科植入物疗效所必需的成骨细胞功能。(C)2006年威利期刊公司。
Many engineers and surgeons trace implant failure to poor osseointegration (or the bonding of an orthopedic implant to juxtaposed bone) and/or bacteria infection. By using novel nanotopographies, researchers have shown that nanostructured ceramics, carbon fibers, polymers, metals, and composites enhance osteoblast adhesion and calcium/phosphate mineral deposition. However, the function of bacteria on materials with nanostructured surfaces remains largely uninvestigated. This is despite the fact that during normal surgical insertion of an orthopedic implant, bacteria from the patient's own skin and/or mucosa enters the wound site. These bacteria (namely, Staphylococcus epidermidis) irreversibly adhere to an implant surface while various physiological stresses induce alterations in the bacterial growth rate leading to biofilm formation. Because of their integral role in determining the success of orthopedic implants, the objective of this in vitro study was to examine the functions of (i) S. epidermidis and (ii) osteoblasts (or bone-forming cells) on ZnO and titania (TiO2), which possess nanostructured compared to microstructured surface features. ZnO is a well-known antimicrobial agent and TiO2 readily forms on titanium once implanted. Results of this study provided the first evidence of decreased S. epidermidis adhesion on ZnO and TiO2 with nanostructured when compared with microstructured surface features. Moreover, compared with microphase formulations, results of this study showed increased osteoblast adhesion, alkaline phosphatase activity, and calcium mineral deposition on nanophase ZnO and TiO2. In this manner, this study suggests that nanophase ZnO and TiO2 may reduce S. epidermidis adhesion and increase osteoblast functions necessary to promote the efficacy of orthopedic implants. (c) 2006 Wiley Periodicals, Inc.