Promoters and regulatory elements that improve adeno-associated virus transgene expression in the brain
Promoters and regulatory elements that improve adeno-associated virus transgene expression in the brain
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DOI：
10.1016/s1046-2023(02)00227-x
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发表时间：
2002-10-01
期刊：
影响因子：
4.8
通讯作者：
During, MJ
中科院分区：
文献类型：
--
作者：
Fitzsimons, HL;Bland, RJ;During, MJ
自从第一次证明用重组腺相关病毒转导中枢神经系统（CNS）以来，载体生产和启动子强度的改进已经导致转导的细胞数量和每个细胞内的表达水平的显著增加。启动子强度的改善导致从原来的巨细胞病毒（CMV）的启动子对使用混合CMV为基础的启动子和组成型细胞启动子，这篇综述总结和比较不同的启动子和调控元件，已被用于与rAAV作为一个参考，实现高水平的rAAV介导的转基因表达在中枢神经系统。(C)2002 Elsevier Science（美国）。All rights reserved.
Since the first demonstration of central nervous system (CNS) transduction with recombinant adeno-associated virus, improvements in vector production and promoter strength have lead to dramatic increases in the number of cells transduced and the level of expression within each cell. The improvements in promoter strength have resulted from a move away from the original cytomegalovirus (CMV) promoter toward the use of hybrid CMV-based promoters and constitutive cellular promoters, This review summarizes and compares different promoters and regulatory elements that have been used with rAAV as a reference toward achieving a high level of rAAV-mediated transgene expression in the CNS. (C) 2002 Elsevier Science (USA). All rights reserved.