Molecular mediators of Porphyromonas gingivalis-induced T-cell apoptosis
Molecular mediators of Porphyromonas gingivalis-induced T-cell apoptosis
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DOI：
10.1034/j.1399-302x.2002.170404.x
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发表时间：
2002-08-01
影响因子：
--
通讯作者：
Taylor, JJ
中科院分区：
文献类型：
--
作者：
Harris, JI;Russell, RRB;Taylor, JJ
牙龈卟啉单胞菌产生毒力因子，其可以改变宿主免疫应答的分子和细胞组分。在目前的工作中，我们研究了牙龈卟啉单胞菌的特异性毒力因子在诱导Jurkat T细胞凋亡中的作用。牙龈卟啉单胞菌培养上清液模拟丁酸对T细胞凋亡的作用，并且这种作用与组蛋白H4乙酰化的增加相关。在实验中排除了蛋白酶的作用，这些实验证明蛋白酶抑制剂和使用蛋白酶合成缺陷的牙龈卟啉单胞菌突变体对该系统中T细胞凋亡的刺激都没有任何影响。
Porphyromonas gingivalis produces virulence factors which can modify the molecular and cellular components of the host immune response. In the present work we investigated the role of specific virulence factors from P. gingivalis in the induction of apoptosis in Jurkat T cells. P. gingivalis culture supernatants mimicked the effect of butyric acid on T-cell apoptosis and this effect was associated with an increase in histone H4 acetylation. A role for proteases was excluded in experiments which demonstrated that neither protease inhibitors nor use of P. gingivalis mutants defective in protease synthesis had any effect on the stimulation of T-cell apoptosis in this system.