Detection and quantification of Fusarium oxysporum f. sp. cepae in onion plant.
Detection and quantification of Fusarium oxysporum f. sp. cepae in onion plant.
复制标题
尖孢镰刀菌 f 的检测和定量。
DOI：
10.1007/s10327-015-0589-8
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发表时间：
2015
影响因子：
1.2
通讯作者：
S.
中科院分区：
文献类型：
--
作者：
Sasaki;K.;Nakahara;K.;Shigyo;M.;Tanaka;S.;Ito;S.
尖孢镰刀菌从洋葱基腐病中分离到的洋葱孢囊线虫（FOC）与洋葱孢囊线虫（FocSIX3）的SIX3基因（FolSIX3）具有91.4%的同源性。尖孢菌属番茄斑点病菌（FOL）。根据FocSIX3和FolSIX3的核苷酸序列差异，设计了一对用于洋葱FOC检测的引物（P1）。P1仅从洋葱致病性FOC分离物中扩增出一个106 bp长的DNA片段，而从大葱或F.应用P1荧光定量PCR技术对洋葱根和基盘上的FOC进行了定量分析。
Fusarium oxysporumf. sp.cepae(FOC) isolated from onion basal rot possessed aSIX3homolog (FocSIX3) with 91.4 % identity to theSIX3gene (FolSIX3) ofF. oxysporumf. sp.lycopersici(FOL). A primer pair (P1) was designed to detect onion FOC based on differences in nucleotide sequences betweenFocSIX3andFolSIX3. P1 amplified a 106-bp-long DNA segment only from FOC isolates pathogenic to onions, but not from FOC isolated from either Welsh onion orF. oxysporumstrains belonging to formae speciales other thancepae.Real-time quantitative PCR with P1 was developed to quantify onion root- and basal-plate-colonizing FOC.