Accumulation of HDMBOA-Glc is induced by biotic stresses prior to the release of MBOA in maize leaves
Accumulation of HDMBOA-Glc is induced by biotic stresses prior to the release of MBOA in maize leaves
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DOI：
10.1016/j.phytochem.2004.09.006
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发表时间：
2004-11-01
期刊：
影响因子：
3.8
通讯作者：
Iwamura, H
中科院分区：
文献类型：
--
作者：
Oikawa, A;Ishihara, A;Iwamura, H
研究了生物胁迫对玉米叶片苯并恶嗪酮（Bxs）含量的影响。接种南方玉米叶枯病病原菌双星（Bipolaris maydis）后，第3片叶片上2-羟基-4,7-二甲氧基-1,4-苯并恶嗪-3- 1糖苷（HDMBOA-Glc）含量增加，接种48 h后达到最大值。接种弱致病性弯孢菌和非致病性交替曲孢菌也能引起HDMBOA-Glc的积累，同时，maydis、C . lunata和A. alternata的培养滤液也显示真菌积累了激发活性化合物。此外，maydis感染诱导形成深褐色病变，其中最丰富的bx相关化合物是6-甲氧基-2-苯并恶唑啉酮（MBOA）。后者由DIMBOA和HDMBOA降解形成，而HDMBOA- glc在周围绿色组织中含量最多。在bx相关化合物中，MBOA对麦冬花、月桂花和花青花的分生孢子萌发和胚管生长的抑制作用最强。除真菌感染外，粘虫幼虫的取食也导致了HDMBOA-Glc的积累。这些发现讨论了与将DIMBOA-Glc转化为HDMBOA-Glc的可能的生态相关性。(C) 2004 Elsevier Ltd.版权所有。
The effects of biotic stresses on the contents of benzoxazinones (Bxs) were investigated in maize leaves. When the causal agent of southern corn leaf blight, Bipolaris maydis, was inoculated on the third leaf, the amount of 2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one glucoside (HDMBOA-Glc) increased, reaching a maximum level 48 h after inoculation. The inoculation of weakly pathogenic Curvularia lunata and non-pathogenic Alternaria alternata also resulted in accumulation of HDMBOA-Glc, and filtrates of the cultures of B. maydis, C lunata and A. alternata also showed the accumulation of elicitor-active compounds by the fungi. Furthermore the infection of B. maydis induced formation of dark brown lesions, where most abundant Bx-related compound was 6-methoxy-2-benzoxazolinone (MBOA). The later is formed by degradation of DIMBOA and HDMBOA, whereas HDMBOA-Glc was most abundant in the surrounding green tissues. Among the Bx-related compounds, MBOA exhibited the strongest inhibition of the germination of the conidia and of the growth of germ tubes of B. maydis, C lunata and A. alternata. In addition to fungal infection, the feeding by rice armyworm, larvae resulted in HDMBOA-Glc accumulation. These findings are discussed in relation to the possible ecological relevance of the conversion of DIMBOA-Glc into HDMBOA-Glc. (C) 2004 Elsevier Ltd. All rights reserved.